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Datasheet
Goat Anti-Mouse IgG (H+L) PE conjugated secondary antibody
Cat.No 20103
Description Anti-Mouse IgG (H+L) PE Conjugate
Unit 500 Tests
Price(USD) 145.00
MSDS
Catalog No
:
20103
Contents
:
250 ul in PBS containing 0.05% sodium azide and 15 mg BSA/ml. Sodium azide is highly toxic.
Isotype
:
Goat Ig
Clone
:
N/A
Purification
:
purified
Species
:
Mouse
Predicted React
:
Mouse
Host
:
Goat
Application
Flow (Cell Surface): 0. 5 ul/one million cells (0.25ug) Flow (Intracellular): 0. 5 ul/one million cells (0.25ug) Storage Product is light- and temperature-sensitive. Store in the dark at 4°C. Do not freeze.
Background
The antibody was isolated from antisera by a combination of pepsin digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
Antibody/PE ratio: 1:1
Minimal cross-reaction to human, bovine, horse and rabbit.
Application Notes
The antibody conjugate has been tested by FACS analysis at 0.5 ul / one million cells to assure specificity and reactivity. Since applications vary, investigators should determine dilutions appropriate for individual use.
Intracellular flow analysis of TLR5 in human PBMC (monocytes) using 0.5 ug of IMG-664A. Shaded histogram represents cells without antibody; green represents isotype control (BD Biosciences); red represents IMG-664A. Secondary antibody Goat Anti-Mouse IgG PE (Imgenex, cat. 20103).
Product Citations 1. In vivo selection of transduced hematopoietic stem cells and little evidence of their conversion into hepatocytes in vivo. Kanji Yamaguchi, Katsuhiko Itoh, Tomoko Masuda, Atsushi Umemura, Christopher Baum, Yoshito Itoh, Takeshi Okanoue and Jun Fujita. Journal of Hepatology, 45 (5): 681-687 (2006). 2. Gfra1 silencing in mouse spermatogonial stem cells results in their differentiation via the inactivation of RET tyrosince kinase. He Z, M-C Hofmann, and M Dym. Biology of Reproduction 77: 723-733 (2007). Imgenex antibodies cited (mouse sperm): 1. GFRA1 (IMG-192), Figs 1B, 5D (siRNA validated). 2. Rabbit IgG-HRP (20103), Figs 1B, 5D..3. Beta-actin (IMG-5142A), Figs 1B, 5D, 8B&E....
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
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