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Monoclonal Antibody to Mouse IgG1 Isotype Control (Clone MOPC-31C) Alexa Fluor® 647 Conjugate |
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| Catalog No: |
20109AF647 |
| Contents: |
100 ug in 200 ul of PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
MOPC-31C |
| Purification: |
Affinity Chromatography |
| Host: |
Mouse |
| Application: |
Multiple
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| Storage: |
Product is light- and temperature-sensitive. Store in the dark at 4°C. Do not freeze. |
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Background
A mouse isotype control is an antibody of the same class
(isotype) of immunoglobulin as the specific mouse antibody, but is either
myeloma-derived antibody of unknown specificity, or is raised against an antigen
that is presumed not to be present on or in the cells under study. Isotype
control antibodies are used for the estimation of non-specific binding of target
primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse
isotype controls including fluorescene conjugates and azide-free
formats. |
Application Notes
The MOPC-31C hybridoma cell line was grown and tissue culture supernatant was purified using affinity chromatography.
Click here to see a list of additional mouse isotype control antibodies. |
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Cell surface flow cytometric analysis of CD2 in 10^6 human lymphocytes using 10 ul (0.25 ug) of IMG-6296AF647. Shaded histogram represents cells without antibody; green represents isotype control (IMGENEX, 20109AF647); red represents anti-CD2 antibody. IMGENEX's cell surface flow kit (IMGENEX, 10084K) was used for this test (cells were not fixed for testing). |
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Reference
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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