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Rabbit IgG Isotype Control |
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| Catalog No: |
20304 |
| Contents: |
100 ug of rabbit isotype control in 200 ul of PBS containing 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Purification: |
Protein G Chromatography |
| Host: |
Rabbit |
| Application: |
Flow & IHC: please see Patole et al. (2006) for details
Multiple
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| Storage: |
Aliquot and store rabbit isotype control at -20°C. |
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Background
A rabbit isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of rabbit isotype controls including fluorescene conjugates and azide-free formats. |
Application Notes
Naive rabbit sera was purified using affinity chromatography.
Click here to see a list of additional rabbit isotype control antibodies. |
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Intracellular FACS analysis of mouse TLR6 (IMG-527) polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti rabbit FITC (Imgenex, 20302) was used as secondary. |
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Reference
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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