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Monoclonal Antibody to CD1a (Clone 201B5.08) Alexa 647 Conjugated |
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| Catalog No: |
DDX0081A647-50 |
| Contents: |
Antibody in PBS 50% glycerol |
| Isotype: |
IgG1 |
| Clone: |
201B5.08 |
| Purification: |
QMA Hyper D ion exchange chromatography) |
| Species: |
Human |
| Host: |
Mouse |
| Supplier: |
Dendritics |
| Market Availability: |
North America (United States & Canada) |
| Application: |
Flow (Cell Surface): 5-20ug/ml*
IF/ICC: 5-20ug/ml* |
| Storage: |
Aliquot and store at -20°C, protected from light. Contains no preservative; KEEP STERILE; AVOID REPEATED FREEZE/THAW CYCLES. |
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Background
CD1 family molecules have a homologous structure to MHC class I. They are monomorphic proteins of 50 kDa non-covalently associated with b2-microglobulin. Human CD1a molecules are divided in 2 groups. Group 1 includes CD1a, CD1b, and CD1c, while group 2 consists of the CD1d molecule. CD1a is mainly expressed at the cell-surface and in recycling vesicles. CD1a occurs in Birbeck granules of Langerhans cells and plays a role in presentation of non-peptide antigens. CD1a is expressed in vitro on CD34+-generated DCs and on monocyte-derived DCs. (Burdin N. et al., 1999 Curr Opin Immunol, 11:326-31) |
Antigen
in vitro derived human DCs(GMCSF + TNFa) |
Application Notes
*This monoclonal antibody may be used between 5-20 ug/ml *Optimal dilution should be determined by each laboratory for each application *Coupled antibody: to maintain RT before using |
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Surface Flow cytometryCD1a expression onCD34+-derived DCs ( day 12 of culture) |
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Reference
1. Burdin N. et al., 1999 Curr Opin Immunol, 11:326-31
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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