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Monoclonal Antibody to CD207/Langerin (Clone 808E10.01) |
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| Catalog No: |
DDX0360-50 |
| Contents: |
Antibody in Tris-NaCl pH 8 |
| Isotype: |
IgG1 |
| Clone: |
808E10.01 |
| Purification: |
QMA Hyper D ion exchange chromatography) |
| Species: |
Human, Mouse, Rat |
| Host: |
Mouse |
| Supplier: |
Dendritics |
| Market Availability: |
North America (United States & Canada) |
| Application: |
FA (Neutralization): 5-20 µg/ml*
Flow (Cell Surface): 5-20ug/ml*
IHC (frozen): 5-20ug/ml* |
| Storage: |
Aliquot and store at -20°C, protected from light. Contains no preservative; KEEP STERILE; AVOID REPEATED FREEZE/THAW CYCLES. |
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Background
Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 2002; J. Immunol., 168 : 782-779 ; Douillard P et al, 200; J. Invest. Dermatol., 125:983–994). |
Antigen
human Langerin-transfected eukaryotic cells |
Application Notes
Langerin-gp120 blocking protocol: Mock- and Langerin-transfected CHO cells are incubated with biotin-labelled gp160 (5ug/ml) and clone 808E10.01 at (1 to 10ug/ml) in PBS 45 min. at RT. Cells were washed in PBS 5min at 1600rpm, and stained with Streptavidin-PE 30 min at 4°C (DAKO). A last PBS wash is performed before Flow cytometry analysis. *This monoclonal antibody may be used between 5-20 ug/ml. *Optimal dilution should be determined by each laboratory for each application. *Coupled antibody: to maintain RT before use. |
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Blocking HIV-gp120 (808E10.01 anti-langerin clone). Non -blocking anti-langerin clone |
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Human skin frozen section: Langerhans cells staining with 808E10.01-HRP. |
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Reference
1. Valladeau J et al, 2002 J Immunol, 168:782-792
2. Douillard P et al, 2005 J Invest Dermatol, 125:983–994
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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