|
|
|
| |
Monoclonal Antibody to CD207/Langerin (Clone 310F7.02/HD26) |
|
|
| |
|
|
| |
|
| Catalog No: |
DDX0361-50 |
| Contents: |
Antibody in Tris-NaCl pH 8 |
| Isotype: |
IgG1 |
| Clone: |
310F7.02/HD26 |
| Purification: |
Purified using a QMA Hyper D ion exchange column |
| Species: |
Human, Mouse, Rat |
| Host: |
Mouse |
| Supplier: |
Dendritics |
| Market Availability: |
North America (United States & Canada) |
| Application: |
Flow (Intracellular): 5-20 ug/ml*
IHC (Bouin): 5-20 ug/ml* |
| Storage: |
Aliquot and store at -20°C. Contains no preservative; KEEP STERILE;AVOID REPEATED FREEZE/THAW CYCLES. |
|
 |
|
|
|
|
|
|
Background
Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. |
Antigen
Recombinant Langerin |
Application Notes
*Optimal dilution should be determined by each laboratory for each application |
Genebank Info (Protein)
NP_056532 |
| |
|
Human skin paraffin section: staining with 310F7.02 +goat anti mouse-HRP. |
|
|
|
|
Flow (intracellular) analysis of saponin permeabilized (0.3%) CHO-Langerin transfected cells using DDX0361 (clone 310F7.02) at 5ug/ml (A), and 1ug/ml (B). CHO-Langerin transfected cells were incubated with DDX0361 for 30 min at 4°C, washed in PBS-Saponin, followed by anti-mouse FITC (DAKO) secondary reagent (1/20 dilution) for 5 min. |
|
|
|
|
|
| - |
 |
|
Reference
1. Valladeau J et al, 2002 J Immunol, 168 : 782-792
2. Douillard P et al, 2005 J Invest Dermatol, 125: 983–994
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
