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Monoclonal Antibody to pDC/IPC (Clone 120G8.04) Alexa 488 Conjugated |
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| Catalog No: |
DDX0390A488-50 |
| Contents: |
Antibody in PBS 50% glycerol |
| Isotype: |
IgG1, Kappa |
| Clone: |
120G8.04 |
| Purification: |
Purified using a QMA Hyper D ion exchange column |
| Species: |
Mouse |
| Host: |
Rat |
| Supplier: |
Dendritics |
| Market Availability: |
North America (United States & Canada) |
| Application: |
Flow (cell surface): 1-10ug/ml*
IF/ICC: 1-10ug/ml* |
| Storage: |
Aliquot and store at -20°C, protected from light. Contains no preservative; KEEP STERILE; AVOID REPEATED FREEZE/THAW CYCLES. |
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Background
We generated rat monoclonal antibody (mAb) that recognizes mouse plasmacytoid dendritic cells (pDCs). This antibody, named 120G8, stains a small subset of CD11c(low) spleen cells with high specificity. This population produces high amounts of IFN-alpha upon in vitro viral stimulation. Both ex vivo- and in vitro-derived 120G8(+) cells display a phenotype identical with that of mouse pDCs (B220(high)Ly6C(high)Gr1(low)CD11b(-)CD11c(low)). Mice treated with 120G8 mAb are depleted of B220(high)Ly6C(high)CD11c(low) cells and have a much-reduced ability to produce IFN-alpha in response to in vivo CpG stimulation. MAb 120G8 stains all and only B220(high)Ly6C(high)CD11c(low) pDC in all lymphoid organs. Immunohistochemical studies performed with this mAb indicate that pDC are located in the T cell area of spleen, lymph nodes, and Peyer's patches. Using 120G8 mAb in immunofluorescence studies demonstrates mouse strain- and organ-specific differences in the frequency of pDCs and other DC subsets. |
Antigen
Mouse plasmatocytoid DCs (pDCs) |
Application Notes
Optimal dilution should be determined by each laboratory for each application |
| Genebank Info (Protein): NP_072098 |
| Gene ID: 5132 |
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Depletion in vivo of mouse pDC. |
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FACS sorting of mouse pDCs with 120G8.04/CD11C. |
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Peroxydase labeling using clone 120G8.04 on mouse frozen spleen sections. |
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Reference
1. Asselin-Paturel C et al., 2003 J. Immunol., 172:6466
2. Blasius AI, 2006 J. Immunol, 177:3260
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa FluorŽ is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa FluorŽ dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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