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Monoclonal Antibody to CD127/IL-7R (Clone R34-34) Alexa 647 Conjugated |
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| Catalog No: |
DDX0700A647-50 |
| Contents: |
Antibody in PBS 50% glycerol |
| Isotype: |
Mouse IgG1 |
| Clone: |
R34-34 |
| Purification: |
Purified using a QMA Hyper D ion exchange column |
| Species: |
Human |
| Host: |
Mouse |
| Supplier: |
Dendritics |
| Market Availability: |
North America (United States & Canada) |
| Application: |
Flow (Cell Surface): 5-20 ug/ml* |
| Storage: |
Aliquot and store at -20°C. Contains no preservative; KEEP STERILE;AVOID REPEATED FREEZE/THAW CYCLES. |
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Background
The IL-7R consists of 2 chains, IL-7Ra known as CD127 and common cytokine receptor g chain known as CD132. A 75-to 80kD human IL-7 receptor has been cloned that belongs to hematopoietic cytokine-receptor superfamily. R34-34 raised against human leukemic pre-Bcells recognized a molecule expressed on normal B cell precursors but not on mature B cells. This antibody specifically reverted IL-7 mediated growth inhibition of leukemic BCP (normal B cells precursors) and mature T cells. IL-7Ra expression is dramatically influenced by cytokines and antigens. This IL-7R displays both high and low affinity for its ligand (IL-7). Inhibitory and proliferative effects of IL-7 can be mediated through the same receptor on various lineages. CD4+memory Tcells express high level of IL-7R Subsets that express it generally require it, including progenitors of T and B cells, naïve t cells and memory T cells. |
Antigen
Human leukemic pre-B cells |
Application Notes
*Optimal dilution should be determined by each laboratory for each application
FA (Neutralization): Clone R34-34 has been validated in IL7-binding inhibition assays. Users are encouraged to optimize according to model system
Clone R34.34 confirmed by HCDM/HLDA 2006 workshop as CD127 |
| Genebank Info (Protein): NP_002176 |
| Gene ID: 3575 |
| SwissProt: P16871 |
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Flow cytometric analysis of IL-7R transfected Cos-7 cells using clone R34-34. |
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Flow cytometric analysis of total peripheral blood lymphocytes using clone R34-34. |
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Immunoprecipitation of R34-34 antigen from125I-surface labelled Pre-Alp cells. |
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Reference
1. Pandrau-Garcia D et al, 1994, Blood, 83, 3613
2. Mazzucchelli R et al, Nat. Review Immunol., 2007, 7, 144
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa Fluor® dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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