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Product : Antibody >> Apoptosis , Neurobiology , Nuclear & Cytoplasmic.. , SUMO >> Cytochrome C
      
 

Monoclonal Antibody to Cytochrome C(Clone 7H8.2C12)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-101A
Cytochrome C Purified
0.1 mg
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Catalog No: IMG-101A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG2b, Kappa
Clone: 7H8.2C12
Purification: Protein G Chromatography
Species: Dog, Drosophila, Honey Bee, Horse, Human, Mammals, Pig, Rabbit
Host: Mouse
Application:
Flow (Intracellular): See Mohr (2004) et al.
IF/ICC: See Yamasaki (2006) et al.
IHC (paraffin): See Fujimara (1998). et al.
Western blot analysis: 0.05-0.5 ug/ml
Storage: Store cytochrome C antibody at 4°C. For long-term storage, store at -20°C.
 

Background
In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3 (2).  Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Antigen
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).

Application Notes
This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).

Genebank Info (Protein): NP_061820
Gene ID: 54205
SwissProt: P99999
 
Cytochrome C Antibody

Western blot analysis using cytochrome C antibody. Human HeLa lysate probed with cytochrome C antibody at 0.1 ug/ml.

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Reference
1. Fernandes-Alnemri, T., Litwack, G., and Alnemri, ES.  J. Biol. Chem. 269: 30761-30764 (1994). PMID: 7983002.
2. Liu, X., Kim, CN, Yang, J., Jemmersen, R., and Wang, X. Cell 86: 147-157 (1996). PMID: 8689682.
3. Zou, H., Henzel, WJ, Liu, X., Lutschg, A., and Wang, X. Cell 90: 405-413 (1997). PMID: 9267021.
4. Jemmersen, R. Johnson, J.G., Burell, E., Taylor, P.S., and Jemkins, M.K.  Eur. J. Immunol. 21: 143-151 (1991). PMID: 1846813.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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