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Product : Antibody >> Apoptosis , Nuclear & Cytoplasmic.. >> Cytochrome C
      
 

Monoclonal Antibody to Cytochrome C (Clone 6H2.B4)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-102A
Cytochrome CPurified
0.1 mg
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Catalog No: IMG-102A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg antibody in 0.2 ml PBS containing 0.05%BSA, 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 6H2.B4
Purification: Protein G Chromatography
Species: Human, Mouse, Rat
Host: Mouse
Application: FA (Neutralization):users should determine optimal conditions.
IHC (frozen): 2-4 ug/ml
IP: 2 ug/1X10^6 cells
Storage: Store antibody at 4C. For long-term storage, store at -20C.
 

Background
In addition to its well-known role in electron-transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death (1). Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade bay activating caspase-3 (2). Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3 (3).

Antigen
Rat cytochrome c was used as immunogen to generate 6H2.B4 hybridoma clone (Goshorn et al, 1991). The antibody recognizes an epitope around residue 62 of rat Cytochrome c, based on copetitive ELISA results (Goshorn et al. 1991).

Application Notes
(1). Clone 6H2.B4 (IMG-102A) recognizes the native form of cytochrome c (Goshorn et al. 1991). Native cytochrome c has an approximate molecular weight of 15 kDa. (2). 6H2. B4 does not appear to recognize the denatured form of cytochrome c. Clone 7H8.2C12 (IMG-101A) is recommended for western blot. (3). 6H2.B4 (IMG-102A) can be used in combination with 7H8.2C12 (IMG-101A) for IP/Western blot analysis of cytochrome c. Please refer to the Figure. (3) Blocking: 6H2.B4 has been shown to inhibit caspase 3 activation when microinjected into cells. Users should determine concentration and conditions for their model systems.

Genebank Info (Protein): NP_061820
Gene ID: 54205
SwissProt: P99999
 
Cytochrome C Antibody

Immunoprecitation/Western blot analysis using cytochrome c antibodies. Two micrograms of cytochrome c antibody clone 6H2.B4 (IMG-102) (lane 1) or control IgG antibody (lane 2) were used to immunoprecipitate cytochrome c from 1 x 10^6 HeLa cells. The immunoprecipitated protein was resolved by SDS-PAGE and probed with cytochrome antibody clone 7H8.2C12 (IMG-101A, 2 ug/ml).

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Reference
1. Liu, X., Kim, CN, Yang, J., Jemmersen, R., and Wang, X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell 86: 147-157 (1996).
2. Fernandes-Alnemri, T., Litwack, G., and Alnemri, ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme J. Biol. Chem. 269: 30761-30764 (1994).
3. Zou, H., Henzel, WJ, Liu, X., Lutschg, A., and Wang, X. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 90: 405-413 (1997).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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