PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Inflammation , NF-kB Pathway , TNF Family & Death Re.. >> OPG
      
 

Monoclonal Antibody to Human Osteoprotegerin (OPG)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-103A
OPGPurified
0.1 mg
 --
 
Catalog No: IMG-103A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 98A1071
Purification: Protein G Chromatography was used to purify the antibody.
Species: Human
Host: Mouse
Application: Flow (I): 0.2-1 ug/10^6 cells
IF/ICC: see Sandra 2006
IHC (frozen): see Boumans 2012 for details.
IHC (paraffin): 5 ug/ml + multiple Product Citations
IP: see Sandra 2006
Western blot analysis: 1-3 µg/ml
Storage: Store antibody at 4°C. For long term storage, store antibody at -20°C.
 

Background
Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily (1). As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease.

Antigen
A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human OPG was used as immunogen for this antibody. The specificity was confirmed on recombinant OPG by western blot analysis.

Application Notes
The OPG antibody, clone 98A1071, detects all forms of OPG including monomeric, dimeric and OPG bound to RANKL (Vandooren, 2008) as well as OPG bound to TRAIL (Sandra, 2006).

Genebank Info (Protein): NP_002537
Gene ID: 4982
SwissProt: O00300
 
OPG Antibody WB

Western blot analysis of Daudi lysate using the OPG antibody at 1 ug/ml.

OPG Antibody FACS

Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary (20103) and IMGENEXs IC Flow kit (10083K).

OPG Antibody IHC

Formalin-fixed, paraffin-embedded normal human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

-

Reference
1. Bucay N, Sarosi I, Dunstan CR, Morony S et al. Osteoprotegerin-deficient mice develop early onset osteoporesis and arterial calcification. Genes & Develop. 12: 1260-1268 (1998).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways