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Monoclonal Antibody to Human Osteoprotegerin (OPG) |
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| Catalog No: |
IMG-103A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
98A1071 |
| Purification: |
Protein G Chromatography was used to purify the antibody. |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
Flow (I): 0.2-1 ug/10^6 cells
IF/ICC: see Sandra 2006
IHC (frozen): see Boumans 2012 for details.
IHC (paraffin): 5 ug/ml + multiple Product Citations
IP: see Sandra 2006
Western blot analysis: 1-3 µg/ml |
| Storage: |
Store antibody at 4°C. For long term storage, store antibody at -20°C. |
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Background
Osteoprotegerin (OPG) is a secretory glycoprotein belonging to TNF receptor (TNFR) superfamily (1). As the name implies, it protects bone. Unlike other TNFRs it lacks a transmembrane domain and lacks any apparent cell associated signals. OPG consists of 401 amino acid with molecular mass of approximately 55 kD as a monomer and 110 kD as a disulfide-linked dimer. High levels of OPG mRNA has been detected in lung, heart, kidney, and placenta1. Recombinant OPG blocks osteoclastogenesis in vitro and increases bone density in vivo. Targeted deletion of OPG in mice results in severe early-onset of osteoporosity. These mice also exhibit increase in vascular calcification in the aorta and renal arteries suggesting that it may also play a role in degenerative arterial disease. |
Antigen
A peptide corresponding to the amino acids 20-37 TQETFPPKYLHYDEETSH of human OPG was used as immunogen for this antibody. The specificity was confirmed on recombinant OPG by western blot analysis. |
Application Notes
The OPG antibody, clone 98A1071, detects all forms of OPG including monomeric, dimeric and OPG bound to RANKL (Vandooren, 2008) as well as OPG bound to TRAIL (Sandra, 2006). |
Genebank Info (Protein)
NP_002537 |
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Western blot analysis of Daudi lysate using the OPG antibody at 1 ug/ml. |
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Intracellular staining of 10^6 HeLa cells using 0.2 ug of OPG antibody, 0.5 ul of PE secondary (20103) and IMGENEXs IC Flow kit (10083K). |
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Formalin-fixed, paraffin-embedded normal human breast tissue stained with OPG antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. |
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Reference
1. Bucay N, Sarosi I, Dunstan CR, Morony S et al. Osteoprotegerin-deficient mice develop early onset osteoporesis and arterial calcification. Genes & Develop. 12: 1260-1268 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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