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Product : Antibody >> Apoptosis , Cancer Antibodies , TNF Family & Death Re.. , Transcription Factors >> TRAIL/Apo-2 Ligand
      
 

Polyclonal Antibody to human TRAIL/Apo-2 Ligand

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-110-2
TRAIL/Apo-2 Ligand Purified
0.1 mg
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Catalog No: IMG-110-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.2 ml PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 1-2 g/ml
Storage: Store at 4C.
Recommended
Positive Control:
HeLa
 

Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) (1,2). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL (3-6). Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

Antigen
This polyclonal antibody was raised against a peptide corresponding to amino acids 261-277 of human TRAIL.

Application Notes
A32 kDa band, which represents a membrane bound form of TRAIL, should be observed.

Genebank Info (Protein): NP_003801
Gene ID: 8743
 
TRAIL/Apo-2 Ligand Antibody

Western blot analysis of 10 g of total cell lysate from HeLa cells with IMG-110.

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Reference
1. Wiley SR, Schooley K, Smolak PJ, Din WS, Huang CP, Nicholl JK, Sutherland GR, Smith TD, Rauch C, Smith CA, et al. Identification and characterization of a new member of the TNF family that induces apoptosis.Immunity 3:673-682 (1995).
2. Pitti RM; Marsters SA; Ruppert S; Donahue CJ; Moore A; Ashkenazi A. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J. Biol. Chem. 271:12687-90 (1996).
3. Pan G; O'Rourke K; Chinnaiyan AM; O'Rourke K; Gentz R; Ebner R; Ni J; Dixit VM. The receptor for the cytotoxic ligand TRAIL. Science 276:111-113 (1997).
4. Schneider P, Thome M, Burns K, Bodmer JL, Hofmann K, Kataoka T, Holler N, Tschopp J. TRAIL receptors 1 (DR4) and 2 (DR5) signal FADD-dependent apoptosis and activate NF-kB. Immunity 7:831-836 (1997).
5. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. An antagonist decoy receptor and a death domain-containing receptor for TRAIL. Science 277:815-818 (1997).
6. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science 277:818-821 (1997).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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