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Product : Antibody >> Apoptosis , Cancer Antibodies , Neurobiology , TNF Family & Death Re.. >> DAXX
      
 

Polyclonal Antibody to Daxx

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-112-1
DAXXPurified
0.05 mg
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Catalog No: IMG-112-1
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Contents: 0.05 mg of purified antibody in PBS containing 0.02% sodium azide.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Predicted React: New World monkey, Mouse
Host: Rabbit
Application: Western blot analysis: 1:500 to 1:1000
Storage: Store at 4C.
Recommended
Positive Control:
HeLa
 

Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. A novel DD-containing molecule was recently cloned from mouse, human and monkey and designated Daxx (1,2). Daxx binds specifically to the Fas death domain and enhances Fas induced apoptosis and activates the Jun N-terminal kinase (JNK) pathway. Daxx is widely expressed in fetal and adult human and mouse tissues indicating its important function in Fas signaling pathways (1,2).

Antigen
Rabbit anti-Daxx (CT) polyclonal antibody was raised against a peptide corresponding to amino acid 722 to 740 at C-terminus of human Daxx (1,2).

Application Notes
A120 kDa major band should be observed. The sequences of immunogenic peptide are identical among human, monkey and mouse origins of Daxx (1,2), therefore, this antibody should recognize Daxx from all these species.

 
DAXX Antibody

Western blot analysis of 10 g of total cell lysate from HeLa cells with anti-Daxx (CT) at 1:1000 dilution.

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Reference
1. Yang X, Khosravi-Far R, Chang HY, Baltimore D. Daxx, a novel Fas-binding protein that activates JNK and apoptosis. Cell 1997;89:1067-1076.
2. Kiriakidou M, Driscoll DA, Lopez-Guisa JM, Strauss JF 3rd. Cloning and expression of primate Daxx cDNAs and mapping of the human gene to chromosome 6p21.3 in the MHC region. DNA Cell Biol 1997;16:1289-1298.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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