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Monoclonal antibody to TRF2 (TTAGGG repeat binding factor 2) |
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| Catalog No: |
IMG-124A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
4A794.15 |
| Purification: |
Protein G Chromatography was used to purify the antibody |
| Species: |
Human, Mouse, Muntjac (deer), Rat |
| Host: |
Mouse |
| Application: |
ChIP: see Zhang et al, 2008 for antibody use details
ELISA: 2 ug/ml
Flow (Intracellular): 0.1 ug/10^6 cells
ICC: Product Citation #14
IF/ICC: For additional details, please see Product Citations denoting immunofluorescence
IHC (frozen): 5 ug/ml
IP: 2 ug/10^6 cells
Western blot analysis: 2-4 ug/ml |
| Storage: |
Store antibody at 4°C. For long term storage, store at -20°C. |
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Background
TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3). |
Antigen
Full-length TRF2 protein was used as immunogen for the antibody. |
Application Notes
1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe et al (2000) described the doublet as 65 and 69 kDa using clone 4A794.15 (IMG-124A). However, observed molecular weights could vary depending on molecular weight standards used and gel conditions.
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Genebank Info (Protein)
NP_005643 |
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Western blot analysis of TRF2 in human Jurkat cell lysate using TRF2 antibody at 2 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of TRF2 antibody. The shaded histogram represents cells alone, blue represents isotype control and red represents IMG-124A, anti-TRF2. IMGENEX's intracellular flow kit (IMGENEX, 10083K) was used for this test, and an anti-mouse IgG PE conjugated secondary (IMGENEX, 20103). |
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Immunofluorescent staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004). |
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Reference
1. Broccoli, D., et al. Nature Genetics 17:231-239 (1997).
2. Van Steensel, B., et al. Cell 92:401-413 (1998).
3. Karlseder, J., et al. Science 283: 1321-1325 (1999).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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