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Product : Antibody >> Chromatin , IHCProTM , Immunohistochemistry , Telomere Associated Facto.. >> TRF2
      
 

Monoclonal antibody to TRF2 (TTAGGG repeat binding factor 2)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-124A
TRF2 Purified
0.1 mg
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Catalog No: IMG-124A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 4A794.15
Purification: Protein G Chromatography was used to purify the antibody
Species: Human, Mouse, Muntjac (deer), Rat
Host: Mouse
Application: ChIP: see Zhang et al, 2008 for antibody use details
ELISA: 2 ug/ml
Flow (Intracellular): 0.1 ug/10^6 cells
ICC: Product Citation #14
IF/ICC: For additional details, please see Product Citations denoting immunofluorescence
IHC (frozen): 5 ug/ml
IHC (p): 4 ug/ml
IP: 2 ug/10^6 cells
Western blot analysis: 2-4 ug/ml
Storage: Store antibody at 4°C. For long term storage, store at -20°C.
 

Background
TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (1). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3).

Antigen
Full-length recombinant human TRF2 protein was used as immunogen to generate the antibody.

Application Notes
1. TLR2 may be detected as a single band or as a doublet in western blot. Okabe (2000) described the doublet as 65 and 69 kDa using clone 4A794.15 (IMG-124A). However, observed molecular weights could vary depending on molecular weight standards used and gel conditions.

2. The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature.

3. The TRF2 antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M). TRF2 forms missing the B and M domains are often referred to as mutant TRF2. Refer to the published literature for additional information.

4. Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.

Genebank Info (Protein): NP_005643
Gene ID: 7014
SwissProt: Q15554
 
TRF2 Antibody WB

Western blot analysis of TRF2 in human Jurkat cell lysate using TRF2 antibody at 2 ug/ml. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test.

TRF2 Antibody FACS IF

Top: Intracellular flow cytometric analysis of TRF2 in 10^6 human Jurkat cells using 0.1 ug of TRF2 antibody. The shaded histogram represents cells alone, blue represents isotype control and red represents IMG-124A, anti-TRF2.  IMGENEX's intracellular flow kit (IMGENEX, 10083K) was used for this test, and an anti-mouse IgG PE conjugated secondary (IMGENEX, 20103).

Bottom: Immunofluorescent staining of TRF2-bound telomeres in human HeLa cells (Courtesy of Fotiadou, et al, 2004).

TRF2 Antibody IHC

Formalin-fixed, paraffin-embedded transitional cell carcinoma, urinary bladder, stained with TRF2 antibody (4 ug/ml), peroxidase-conjugate and DAB chromogen. Note specific nuclear staining. IMGENEXs tumor/normal adjacent tissue array slide (IMH-343) was used for this test.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Broccoli, D., et al.  Nature Genetics 17:231-239 (1997).
2. Van Steensel, B., et al.  Cell 92:401-413 (1998).
3. Karlseder, J., et al.  Science 283: 1321-1325 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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