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Product : Antibody >> Cytokine & Chemokine , G-Protein Coupled Recepto.. , Stem Cell Markers >> CXCR4 (CXC Chemokine Receptor 4) (NT) 
      
 

Polyclonal Antibody to CXCR4 (NT) Fusin, LESTR, HUMSTR

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-125-2
CXCR4 (CXC Chemokine Receptor 4) (NT) Purified
0.1 mg
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Catalog No: IMG-125-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.1 ml PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human, Mouse
Predicted React: Most Mammals
Host: Rabbit
Application: IF/ICC: 2 ug/ml
Western blot analysis: 1:1000-1:2000
Storage: Store at 4C.
Recommended
Positive Control:
HeLa
 

Background
Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors (1). Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites (10,11).

Antigen
Rabbit anti-CXCR-4 polyclonal antibody was raised against a peptide corresponding to amino acids 1 to 14 (N-terminus) of human CXCR4(3,9). It will cross react with mouse CXCR4.

Application Notes
In HeLa, a 40-45 KDa band is observed.

Genebank Info (Protein): NP_001008540
Gene ID: 7852
SwissProt: P61073
 
CXCR4 (CXC Chemokine Receptor 4) (NT) Antibody

Western blot analysis of HeLa cell lysate with anti-CXCR4 (NT) at 1:2000 dilution.

CXCR4 (CXC Chemokine Receptor 4) (NT) Antibody IMG-125-2

Immunocytochemical analysis of CXCR4 in formalin-fixed HeLa cells with CXCR4 antibody at 2 ug/ml.

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Reference
1. Dimitrov DS. Cell; 91:721-730 (1997).
2. Feng Y et al. Science ;272:872-877 (1996).
3. Berson JF et al. J Virol; 70:6288-6295 (1996).
4. Doranz BJ et al. Cell; 85:1149-1158 (1996).
5. Endres MJ et al. Cell; 87:745-756 (1996).
6. Bleul CC et al. Nature;382:829-833 (1996).
7. Oberlin E et al. Nature; 382:833-835 (1996).
8. Lapham CK et al. Science; 274:602-605 (1996).
9. Leoetscher M et al. J Biol Chem; 269:232-237 (1994).
10. Brelot A et al. J Virol; 71:4744-954751 (1997).
11. Lu Z et al. Proc Natl Acad Sci USA;94:6426-6431 (1997).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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