Product Citations
1. p38-dependent marking of inflammatory genes for increased NF-kB recruitment. Saccani S, S Pantano, G Natoli. Nature Immunology 3:69-75 (2002).
WB (human purified monocytes): Figs. 4c (bottom panel), 5a (bottom panel), 5c.
2. Two waves of nuclear factor kB recruitment to target promoters. Saccani S, S Pantano, G Natoli. J. Exp. Med. 193:1351-1359 (2001).
WB (mouse LPS-stimulated Raw 264.7 macrophages), Fig. 1.
3. Modulation of NF-kB activity by exchange of dimers. Saccani S, S Pantano, G Natoli. Molecular Cell 11:1563-1574 (2003).
WB (HEK-293 cells transfected with different NF-kB subunits) Fig. 1b
WB (human dendritic cells) Fig. 2a
WB (mutant IkBa adenovirus infected dendritic cells) Fig. 2c
4. PC-SPES: A potent inhibitor of nuclear factor-κB rescues mice from lipopolysaccharide-induced septic shock. Ikezoe T, Y Yang, D Heber, H Taguchi, H P Koeffler. Molecular Pharmacology 64:1521-1529 (2003). Imgenex antibodies cited [WB, LPS-treated RAW 264.7 cells]:
1. IkBa (Ser 32/Ser 36) [IMG-156A], Fig. 3a
2. IkBa [IMG-127A], Fig. 3b
5. Degradation of promoter-bound p65/RelA is essential for the prompt termination of the nuclear factor kB response. Saccani S, I Marazzi, A A Beg, G Natoli. J. Exp. Med., 200:107-113 (2004).
WB (TNF-a stimulated WT and IkBa-/- NIH 3T3), Fig. 1a
Note: IkBa (IMG-127A) recognized the IkBa wildtype but not the IkBa-/-, as expected
WB (p65 transfected HEK-293 cells), Fig. 2a
WB (NIH 3T3), Figs. 4a, 4d
IP (p65 transfected 293T cells), Fig. 2b
Note: IkBa (IMG-127A) co-IP'd p65
6. Andrographolide attenuates inflammation by inhibition of NF-kB activation through covalent modification of reduced cysteine 62 of p50. Xia Y F, B Q Ye, Y D Li, J G Wang, X J He, X Lin, X Yao, D Ma, A Slungaard, R P Hebbel, N S. Key, J Geng. Journal of Immunology 173:4207-4217 (2004).
WB (TNF-a treated 293 cells), Fig 6a
7. Differential effects of NF-kB on apoptosis induced by DNA-damaging agents: the type of DNA damage determines the final outcome. Strozyk E, B Pöppelmann, T Schwarz, D Kulms. Oncogene 25:6239-6251 (2006).
WB (human oral epithelial carcinoma cell line KB) Figs. 1a, 5b, 5d
8. NF-kB Is required for apoptosis prevention during herpes simplex virus type 1 infection. Goodkin M L, A T Ting, J A Blaho. Journal of Virology, 77:7261-7280 (2003).
WB (mouse HEp-2 cells and clonal CD-14 transfected cells treated with either TNF-a or TNF-a plus CHX), Fig. 9a
WB (mouse HEp-2 cells treated with TNF-a plus CHX and mutant IkBaDN cells treated with TNF-a), Fig. 9b
WB (mouse mutant HEp-2 and IkBaDN cells infected with HSV-1(F)), Fig. 10b
WB (mouse mutant HEp-2 and IkBaDN cells infected with HSV-1(KOS1.1)), Fig. 10d
9. The IkB kinase is a key factor in triggering influenza A virus-induced inflammatory cytokines production in airway epithelial cells. Bernasconi D, C Amici, S L Frazia, A Ianaro, M G Santoro. Journal of Biological Chemistry 280:24127-24134 (2005).
WB (WSN-infected A549 human pulmonary cells), Fig. 1a bottom
10. Selected Toll-like receptor agonist combinations synergistically trigger a T helper type 1−polarizing program in dendritic cells. Napolitani G, A Rinaldi, F Bertoni, F Sallusto, A Lanzavecchia. Nature Immunology 6:769-776 (2005).
WB (human MoDC stimulated with LPS, R848, and poly(I:C)), Fig. 8b
11. B Cell maturation antigen, the receptor for a proliferation-inducing ligand and B Cell-activating factor of the TNF family, induces antigen presentation in B Cells. Yang M, H Hase, D Legarda-Addison, L Varughese, B Seed, A T. Ting. Journal of Immunology, 175:2814-2824 (2005).Imgenex antibodies cited [WB]:
1. IkBa Ser 32/Ser 36 [IMG-156A], (A20 B cells stimulated with APRIL), Fig. 2c
2. IkBa [IMG-127A], (A20 cells stimulated with anti-TNFR2), Fig. 6a
12. A cyanobacterial LPS antagonist prevents endotoxin shock and blocks sustained TLR4 stimulation required for cytokine expression Macagno A, M Molteni, A Rinaldi, F Bertoni, A Lanzavecchia, C Rossetti, F Sallusto. J. Exp. Med., 203:1481-1492 (2006).
WB (DCs stimulated with LPS and treated with CyP), Figs. 4a, 6e
13. NF-kB and COX-2 during oral tumorigenesis and in assessment of minimal residual disease in surgical margins. Santhi W S, P Sebastian, B T Varghese, O Prakash, M R Pillai. Experimental and Molecular Pathology 81:123-130 (2006). Imgenex products used (human):
1. IkBa [IMG-127A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1
IHC paraffin analysis (normal oral mucosa, leukoplakia, and oral cancer), Fig. 2
IHC paraffin (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig 6
IHC paraffin analysis (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 7
2. p65 [IMG-150A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1
IHC paraffin analysis (normal oral mucosa, leukoplakia, and oral cancer), Fig. 2
IHC paraffin (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 6
IHC paraffin analysis (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 7
3. p50 [IMG-5812A]: IHC paraffin (normal oral mucosa, leukoplakia, and oral cancer), Fig. 1
IHC paraffin analysis (normal oral mucosa, leukoplakia, and orla cancer), Fig. 2
IHC paraffin (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 6
IHC paraffin amalysis (oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 7
4. NF-kB ELISA [IMK-503]: (normal oral mucosa, leukoplakia, and oral cancer), Fig. 3
(oral cancer: primary tumor, surgical margin, and extra margin tissue), Fig. 8
14. Paeonol attenuates TNBS-induced colitis by inhibiting NF-κB and STAT1 transactivation. Ishiguro K, T Ando, O Maeda, M Hasegawa, K Kadomatsu, N Ohmiya, Y Niwa, R Xavier, H Goto. Toxicology and Applied Pharmacology, 217:35-42 (2006).
WB (mouse colon tissue CW-2 cells stimulated with TNFa in presence of Paeonol) Fig. 5a
15. BAFF and APRIL support chronic lymphocytic leukemia B cell survival through activation of the canonical NF-kB pathway. Endo T, M Nishio, T Enzler, H B Cottam, T Fukuda, D F James, M Karin, T J Kipps. Blood 109:703-710 (2007).
WB (human CLL B cells cultured with rhBAFF or rhAPRIL), Fig. 2b
WB (CLL cells transfected with HA-tagged IkBaSR or empty pcDNA3 vector stimulated with rhBAFF), Fig. 5a
16. Targeting the NF-κB signaling pathway in Notch1-induced T-cell leukemia. T Vilimas, J Mascarenhas, T Palomero, M Mandal, S Buonamici, F Meng, B Thompson, C Spaulding, S Macaroun, M L Alegre, B L Kee, A Ferrando, L Miele, I Aifantis. Nature Medicine 13, 70-77 (2007).
IP (CEM cells treated with Z-Leu-Leu-Nle-CHO and LY411575), Fig. 2d
WB (CEM cells IP'd with IKK antibody or Notch1-IC antibody), Figs. 2e, 2f
17. DHMEQ, a novel nuclear factor-κB inhibitor, induces selective depletion of alloreactive or phytohaemagglutinin-stimulated peripheral blood mononuclear cells, decreases production of T helper type 1 cytokines, and blocks maturation of dentritic cells. Nishioka C, T Ikezoe, Y Jing, K Umezawa, A Yokoyama. Immunology 124:198-205 (2008).
WB (acute lymphoblastic T-cell leukemia Jurkat cells pretreated with DHMEQ and exposed to phytohaemagglutinin), Fig. 4.
18.
Suppression of NF-kB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes; Implications for the treatment of osteoarthritics. Shakibaei M, T John, G Schulze-Tanzil, I Lehmann, A Mobasheri. Biomedical Pharmacology 73:1434-1445. WB: primary cultures of human chondrocytes isolated from articular cartilage.
Imgenex antibodies cited for WB (primary cultures of human chondrocytes isolated from articular cartilage):
1. p65 (IMG-512), Fig 3A and B 2. IkBa (IMG-127A), Fig 3A-C 3. Phospho-IkBa (IMG-156A), Fig 3A and B 4. Phospho-Akt (IMG-187A), Fig 5
19. Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kB signaling pathway. Harikumar K, B Sung, M Pandey, S Guha, S Krishnan, B Aggarwal. Mol Pharma 77: 818-827 (2010). The following monoclonal antibodies were used in western blot; human chronic myeloid leukemia KBM-5 cell line, Fig 4.
1. IKKB/IKK2 (IMG-129A)
2. IKKa/IKK1 (IMG-136A)
3. IKBalpha (IMG-127A)
4. IKKgamma (IMG-324A & IMG-287A)
20. Mild hypothermia inhibits nuclear factor-kB translocation in experimental stroke. Han H, M Karabiyikoglu, S Kelly, R Sobel, M Yenari. J Cereb Blood Flow Metab 23: 589-598 (2003). IMGENEX products cited for western blot in male Sprague-Dawley rats ischemic cortex, ischemic subcortex, contralateral cortex, and contralateral subcortex tissue. The rats were subject to mild and delayed hypothermia.
1. IKK-gamma (IMG-324A): Fig 4A & 4B.
2. IkB-alpha (IMG-127A): Fig 3A & 3C.
3. Antiphosphorylated IkB-alpha (IMG-156A): Fig 3A & 3D.
21. The E3 ubiquitin ligase itch couples JNK activation to TNFa-induced cell death by inducing c-FLIPL turnover. Chang L, H Kamata, G Solinas, J L Luo, S Maeda, K Venuprasad, Y C Liu M Karin. Cell 124:601-613 (2006).Imgenex antibodies cited [WB]:
1. IkBa [IMG-127A], (wt hepatocytes incubated with proteasome inhibitor MG-132 and TNFa or CHX added), Fig. 3a
2. Ubiquitin [IMG-5021]: (wt and Jnk1-/- injected mouse liver with ConA), Fig. 3b
(wt mouse liver injected with LPS and Gal-N and JNK-inhibitor or PBS), Fig. 6e
22. p120-Catenin mediates inflammatory responses in the skin. Perez-Moreno M, M A Davis, E Wong, H A Pasolli, A B Reynolds, E Fuchs. Cell 124:631-644 (2006).
WB (wt and p120 null mouse epidermis), Fig. 7c
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