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Monoclonal Antibody to IKK alpha (Clone 14A231) |
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| Catalog No: |
IMG-136A |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
14A231 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, New World monkey |
| Host: |
Mouse |
| Application: |
Flow (Intracellular): 0.25-0.5 ug/10^6 cells
IHC (paraffin): 5 ug/ml
IP: 1-2 ug/ml
Western blot analysis: 1 ug/ml |
| Storage: |
Store IKK alpha antibody at 4°C for up to six months. For long term storage, aliquot and store antibody at -20°C. |
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Background
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK alpha, IKK beta, and IKK gamma. In vitro, IKK alpha and IKK beta can form homo- and heterodimers that can phosphorylate IkBs at the regulatory serine residues directly. IKK alpha and IKK beta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1) (1), respectively. Targeted disruption of IKK alpha gene in mice results in skin and limb abnormalities and death of newborns (2,3). |
Antigen
This antibody was raised against a His-tagged full-length human IKK alpha protein. |
Genebank Info (Protein)
NP_001269 |
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Western blot analysis of IKK alpha in Daudi cell lysate using IKK alpha antibody at 1 ug/ml. |
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Intracellular staining of HEK293 cells using 0.5 ug of IKK alpha antibody. Green histogram represents the isotype control antibody (20109), red represents the IKK alpha antibody. IMGENEX's intracellular flow kit (10083K) was used for this test, and an anti-mouse IgG1 PE conjugated secondary antibody. |
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Immunohistochemical analysis of IKK alpha in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control antibody (top left) and IKK alpha antibody (bottom left, right) at 5 ug/ml. |
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Reference
1. Delhase, M., Hayakawa, et al. Science 284: 309-313 (1999).
2. Takada, K., et al. Science 284: 313-316 (1999).
3. Hu, Y., Baud, V., Delhase, M., et al. Science 284: 316-320 (1999).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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