Background
TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length (1). TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes (2). TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini (2). The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells (2). Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types (3). |
Application Notes
In a side by side comparison of IMG-148A (TRF2 pAb) and IMG-124A (TRF2 mAb) in Jurkat cells, IMG-124A was stronger and cleaner. It rendered a single band at ~60 kDa, whereas IMG-148A had a weaker band at ~60 kDa as well as a number of uncharacterized additional bands. |
Reference
1. Broccoli, D., Smogorzewska, A., Chong, L., de Lange, T.: Human telomeres contain two distinct myb-related proteins, TRF1 and TRF2. Nature Genetics 17:231-239 (1997).
2. Van Steensel, B., Smogorzewska, A., de Lange T.: TRF2 protects human telomeres form end-end fusions. Cell 92:401-413 (1998).
3. Karlseder, J., Broccoli, D., Dai, Y, Hardy, S., and de Lange, T.. p53- and ATM-dependent apoptosis induced by telomeres lacking TRF2. Science 283: 1321-1325 (1999)
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