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Product : Antibody >> TNF Family & Death Re.. >> SODD
      
 

Polyclonal Antibody to SODD (Silencer of Death Domain)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-152
SODDPurified
0.1 mg
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Catalog No: IMG-152
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.2 ml PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Flow (Cell Surface): See Gehrmann (2005) et al.
Western blot analysis: 2-4 ug/ml
Storage: Store at 4C. For long term storage, store at -20C.
Recommended
Positive Control:
HeLa
 

Background
Several cell surface receptors that are involved in apoptosis contain intracellular death domains and are capable of triggering apoptosis when activated by their respective ligands (1). Due to presence of these death domains and presence of various stimuli in the serum as well as overexpression leading to receptor oligomerization, which leads to constitutive activation of the apoptotic pathway, it has been extremely difficult to generate permanent cell lines overexpressing specific receptors in culture. However, in normal states, this death domain activity is silenced presumably due to presence of other cellular factors. Recently, a DR3 associated protein has been isolated by yeast two-hybrid interaction assays. This cDNA encoding a 457-amino acid protein has been named as SODD (Silencer of Death Domain) (2). SODD also associates with the intracellular domains of TNF-R1, but not with those of TNF-R2, Fas, DR4, or DR5. SODD overexpression suppressed the TNF induced cell death as well as NF-kB activation (2). SODD may act as a negative regulatory protein that is normally associated with death domain of TNF-R1 and probably inhibit self-oligomerization properties of death domain and maintain TNF-R1 in an inactive, monomeric state.

Antigen
This polyclonal antibody was raised against a peptide corresponding to amino acids 443-457 of human SODD protein.

Genebank Info (Protein): NP_004865
Gene ID: 9530
SwissProt: O95429
 
SODD Antibody

Western blot analysis of 30 g of total cell lysate from HeLa cells with anti-SODD (IMG-152) at 2 g/ml.

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Reference
1. Ashkenazi, A. and Dixit, VM. Science 281: 1305, (1998).
2. Jiang,Y., Woronicz, JD, Liu, D., and Goeddel, DV. Science 283: 543-546 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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