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Product : Antibody >> TNF Family & Death Re.. >> DRAK1
      
 

Polyclonal Antibody to DRAK1 (Death Receptor Associated Kinase1)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-157-2
DRAK1 Purified
0.1 mg
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Catalog No: IMG-157-2
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.2 ml PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 2-4 ug/ml
Storage: Store at 4C.
Recommended
Positive Control:
HeLa
 

Background
Protein kinases play important roles in the signal transduction in response to a variety of external stimuli. Recently, several protein kinases have been identified that may also be involved in apoptotic process. Overexpression of a serine/threonine kinase, ZIP kinase can cause the morphological changes typical of apoptosis in NIH 3T3 cells (1). Sanjo et al, have identified two new protein kinases DRAK1 and DRAK2 (Death Receptor Associated Kinase 1 and 2) (1). Both DRAKs and ZIP kinase share significant homology at the amino acid level (1,2). The kinase domains of both DRAKs, ZIP kinase are homologous to DAP (Death-associated protein) kinase, which is involved in the apoptotic signaling induced by interferon-g. Overexpression of DRAKs in NIH 3T3 cells lead to apoptosis. These transfection experiments suggest that C-terminal domain of DRAKs are important for induction of apoptosis.

Antigen
This polyclonal antibody was raised against a peptide corresponding to amino acids 5 to 19 of human DRAK1 protein.

Genebank Info (Protein): NP_004751
Gene ID: 9263
SwissProt: Q9UEE5
 
DRAK1 Antibody

Western blot analysis for DRAK1 using IMG-157 at 2 g/ml dilution against 10 g/lane of MOLT4 (lane 1), A431 (lane 2) and NIH-3T3 (lane 3) cell lysates.

DRAK1 Antibody IMG-157-2

Immunohistochemical staining of Molt4 cells using anti-DRAK1 (NT) at 2 g/ml.

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Reference
1. Sanjo H, Kawai T, and Akira S. DRAKs, novel serine/threonine kinases related to death associated protein kinase that trigger apoptosis. J Biol Chem 273 (44): 29066-29071, 1998.
2. Matsumoto M, Takeda K, Sanjo H, and Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 18 (3): 1642-1651, 1998.


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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