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Product : Antibody >> IHCProTM , Immunohistochemistry , NF-kB Pathway , Signal Transduction , TNF Family & Death Re.. >> TRAF2
      
 

Monoclonal Antibody to TRAF2 (Clone 33A1293)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-162
TRAF2Purified
0.1 mg
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Catalog No: IMG-162
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 33A1293
Purification: Protein G Chromatography was used to purify the antibody
Species: Human
Host: Mouse
Application: IHC (paraffin): 5 ug/ml or see Galen et al (2008).
Western blot analysis: use antibody at 2-4 ug/ml 
Storage: Store TRAF2 antibody at 4°C, stable for six months. For long term storage, aliquot and store antibody at -20°C.
Recommended
Positive Control:
HeLa
 

Background
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF2 is a 502-amino acid protein. Mutagenic studies suggest that the N-terminal RING finger and two adjacent zinc fingers of TRAF2 are required for NF-kB activation, where as interaction with TNFR is mediated through C-terminus domain (5,6). Distinct domains in the N- and -C termini are also required for association with TRAF1 and protein kinase receptor interacting protein (RIP). TRAF2 is involved in cellular resistant to TNF-induced apoptosis. TRAF2 deficient mice appeared normal at birth, however, they die prematurely, probably due to atrophy of thymus, spleen, muscle mass and lack of TRAF2's cytoprotective role.

Antigen
This antibody was raised against amino acids 205 to 222 of human TRAF2.

Genebank Info (Protein): NP_066961
Gene ID: 7186
SwissProt: Q12933
 
TRAF2 Antibody

Western blot analysis of TRAF2 in HeLa lysate using TRAF2 antibody at 2 µg/ml.

TRAF2

Formalin-fixed, paraffin-embedded human transitional cell carcinoma of the urinary bladder (IMH-343) stained with TRAF2 antibody at 5 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Rothe M, Wong SC, Henzel WJ, and Goeddel DV. Cell 78: 681-692 (1994).
2. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, and Baltimore D. Science 267: 1494-1498 (1995).
3. Nakane H, Oshima H, Chung W, Williams-Abbit L, Ware CF, Yagita H, and Okumura K. J. Biol. Chem. 271: 14661-14664 (1996).
4. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996).
5. Takeuchi M, Rothe M, and Goeddel DV. J. Biol. Chem. 271 (33) 19935-1142 (1996).
6. Park YC, Burkitt V, Villa AR, Tong L, and Wu H. Nature 398: 533-538 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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