Monoclonal Antibody to TRAF5 (Tumor Necrois factor (TNF) associated Factor 5)

Background
Tumor necrosis factor (TNF) induced signaling is mediated through association of TNF receptor (TNFR) with adaptor proteins, such as TNF receptor associated proteins (TRAFs). TRAFs form a family of cytoplasmic adapter proteins that mediate signal transduction from many members of the TNF-receptor superfamily (e.g. RANK, CD30, CD40, etc.) and the interleukin-1 receptor. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. Recent molecular cloning studies have lead to identification of six TRAFs (TRAF1-TRAF6) (1-4). TRAF5 is a 558-amino acid protein. TRAF5 is implicated in NF-kB and c-Jun NH(2)-terminal kinase/stress-activated protein kinase activation by members of the TNF receptor superfamily, including CD27, CD30, CD40, and lymphotoxin-beta receptor . Targeted disruption of TRAF5 gene causes defects in CD40-CD27 mediated lymphocyte activation (5).

Antigen
Anti-TRAF5 monoclonal antibody was raised against a fusion protein corresponding to amino acids 77 to 186 of human TRAF5.

Application Notes
In HeLa, a ~64 kDa band is observed.

Genebank Info (Protein)
NP_001029082

Reference
1. Nakano H, Sakon S, Koseki H, Takemori T, Tada K, Matsumoto M, Munechika E, Sakai T, Akiba H, Kobata T, Santee SM, Ware CF, Rennert PD, Taniguchi M, Yagita H., and Okumura K. Proc. Natl. Acad. Sci. USA 96 (17): 9803-9808 (1999).
2. Cao Z, Xiong J, Takeuchi M, and Kurama, T. Nature 383: 443-446 (1996).
3. Rothe M, Wong SC, Henzel WJ, and Goeddel DV. Cell 78: 681-692 (1994).
4. Cheng G, Cleary AM, Ye ZS, Hong DI, Lederman S, and Baltimore D. Science 267: 1494-1498 (1995).
5. Nakane H, Oshima H, Chung W, Williams-Abbit L, Ware CF, Yagita H, and Okumura K. J. Biol. Chem. 271: 14661-14664 (1996).