|
|
|
| |
Polyclonal Antibody to TACE/ADAM17 |
|
|
| |
|
|
| |
|
| Catalog No: |
IMG-176-1 |
| Contents: |
0.05 mg of purified antibody in PBS containing 0.02% sodium azide. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store at 4°C. |
Recommended
Positive Control: |
HeLa or Jurkat |
|
 |
|
|
|
|
|
|
Background
Membrane-bound form of TNF-a is cleaved by a convertase to generate a secreted 17 kD mature protein. TNF-a converting enzyme, TACE, is a membrane bound metalloprotease-disintegrin. It also processes other cell surface proteins, including TNF receptor, TGFa, and alpha cleavage of amyloid protein precursor. |
Antigen
The polyclonal antibody was raised against a peptide corresponding to amino acids 807 to 823 of human TACE. This sequence differs from those of mouse and rat TACE by one amino acid. |
Application Notes
80 to 130 kDa bands are observed, which may represent mature protein, precursor, and glycosylated TACE. |
| Genebank Info (Protein): NP_003174 |
| Gene ID: 6868 |
| |
|
Western blot analysis for TACE using IMG-176 at 1 µg/ml against 10 µg/lane of HeLa (lane 1&3) and Jurkat (lane 2&4) cell lysates in the absence (lane 1&2) and presence (lane 3&4) of blocking peptide. |
|
|
|
|
|
| - |
 |
|
Reference
1. Black RA, et al. Nature 385: 729-733 (1997).
2. Moss ML, et al. Nature 385: 733-736 (1997).
|
|
|
|
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
|
|
|
|
|
