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Product : Antibody >> Chromatin , DNA Methylation & Rep.. >> Dnmt3b
      
 

Monoclonal Antibody to Dnmt 3b (DNA methyltransferase 3b)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-184A
Dnmt3bPurified
0.1 mg
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Catalog No: IMG-184A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 52A1018
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Mouse
Application: ChIP: Please refer to product citations.
IF/ICC: 1-2 ug/ml
IHC (paraffin): Please refer to product citations.
IP: 1-2 ug/ml
Western blot analysis: 2-4 ug/ml
Storage: Store at 4C. For long-term storage, store at -20C.
Recommended
Positive Control:
NIH 3T3 cell lysate
 

Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Antigen
This antibody was raised against bacteria expressed recombinant full-length mouse Dnmt3b. The antibody also recognizes human Dnmt3b.

Application Notes
This antibody does not react with Dnmt3a.

Genebank Info (Protein): NP_787046
Gene ID: 1789
SwissProt: Q9UBC3
 
Dnmt3b Antibody

Western blot analysis for Dnmt3b using IMG-184A at 2 ug/ml against 10 ug of cell lysates of 293 transfected with mouse Dnmt3a (lane 1) and Dnmt3b (lane 2). A protein band of approximate mol. wt. of 110 kDa is only detected in 293 cells transfected with mouse Dnmt3b cDNA.

Dnmt3b Antibody IMG-184A

Western blot analysis of Dnmt3b in A) NIH 3T3 cell lysate, B) recombinant Dnmt3b1 protein (IMGENEX, 40214), and C) 2102Ep human embryonal carcinoma cell lysate using IMG-184A at 4 ug/ml, 0.5 ug/ml, and 3 ug/ml respectively.

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Reference
1. Xie S, Wnag Z, Okano M, Nogami M, Li Y, He WW, Okumura K, and Li E. Gene 236: 87-95 (1999).
2. Okano M, Bell DW, Haber DA,a nd Li E. Cell 99: 247-257 (1999).
3. Reik W, Kelsey G, and Walter J. Nat. Genet 23: 380-382 (1999).
4. Hsieh CL. Mol Cell Biol 19: 8211-8218 (1999).
5. Hansen RS, Wijmenga C, Juo P., Stanek AM, canfield TK, Weemaes CM, and Gartler SM. Proc. Natl. Acad. Sci USA 96: 14412-14417 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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