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Product : Antibody >> NF-kB Pathway , TNF Family & Death Re.. >> RANKL/TRANCE/CD254
      
 

Monoclonal Antibody to RANKL

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-185A
RANKL/TRANCE/CD254Purified
0.1 mg
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Catalog No: IMG-185A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug of antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 12A380
Purification: Protein G Chromatography was used to purify the RANKL antibody
Species: Human, Mouse
Host: Mouse
Application: Flow (Surface): please see Yoneda et al. 2004 for details.
IHC (frozen): please see Yoneda et al. 2004 for details.
Western blot analysis: 1-5 ug/ml
Storage: Store RANKL antibody at 4°C, stable for 6 months. For long-term storage, store antibody at -20°C.
 

Background
TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (4-5). RANKL (TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in DC survival (4). RANKL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (1,2). RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Antigen
A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse RANKL was used as immunogen for this antibody. The antibody also recognizes human RANKL.

Genebank Info (Protein): NP_003692
Gene ID: 8600
SwissProt: O14788
 
RANKL Antibody WB

Western blot analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.

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Reference
1. Anderson, D.M, Maraskovsky, E., Billingsley, W.L., Dougall, W.C., Tometsko, M.E., Roux, E.R., Teepe, M.C., DuBose, R.F., Cosman,     D., and Gaibert, L. Nature 390: 175-179 (1997).
2. Wong, B.R., Josien, R., Lee, S.W., Sauter, B., Li, H-L., Steinman, R.M., and Choi, Y. J. Exp. Med. 186: 2075-2080.
3. Kong, Y-Y., Yoshida, H., Sarosi, I., Tan, H-L., et al. Nature 397: 315-323 (1999).
4. Lacey, D.L. Timms, E,. Tan, H.L., Kelly, M.J. , et al. Cell 93: 165-176 (1998).
5. Hsu, H., Lacey, D.L., Dunstan, C.R., Solovyev, I., et al. Proc. Natl. Acad. Sci. USA 96: 3540-3545 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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