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Monoclonal Antibody to Phospho AKT (Ser473) |
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| Catalog No: |
IMG-187A |
| Contents: |
100 ug of phospho antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
104A282 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Predicted React: |
Chicken, Frog, Zebrafish |
| Host: |
Mouse |
| Application: |
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store phospho AKT antibody at 4°C, stable for 6 months. For long-term storage, store antibody at -20°C. |
Recommended
Positive Control: |
PDGF treated NIH 3T3 |
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Background
AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of AKT is to mediate cell survival. AKT is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 and AKT kinase activity. AKT phosphorylation is critical for it activity. The major phospho sites required for AKT activation have been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2. |
Antigen
This phospho monoclonal antibody was raised against a synthetic peptide containing phospho serines at amino acid residues 473 of human AKT, Accession no. ref|NP_005154.2|. The sequence in this region is identical in human, mouse, chicken, frog and zebrafish. |
Application Notes
Clone 104A282 specifically detects the phospho form of AKT in PDGF treated cells. |
Genebank Info (Protein)
NP_001014432 |
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Western blot analysis of phospho AKT using phospho AKT antibody at 2 ug/ml against untreated (lane 1) and PDGF treated (lane 2) NIH-3T3 lysate. IMGENEX's HRP conjugated secondary antibody (20101) and ECL substrate solution (10087K) were used for this test. |
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Reference
1. Alessi DR, Andjelkovic M, Caudwell B, Cron P, Morrice N, Cohen P, Hemmings BA. Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J. 1996 Dec 2;15(23):6541-51.
2. Paul Ferrigno and Pamela A Silver Regulated nuclear localization of stress-responsive factors: how the nuclear trafficking of protein kinases and transcription factors contributes to cell survival. Oncogene 1 November 1999, Volume 18, Number 45, Pages 6129-6134.
3. Kandel ES, Hay N. The regulation and activities of the multifunctional serine/threonine kinase Akt/PKB. Exp Cell Res. 1999 Nov 25;253(1):210-29.
4. Lewis C. Cantley and Benjamin G. Neel. New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. PNAS 1999 96 (8) 4240-4245; doi:10.1073/pnas.96.8.4240.
5. Franke TF, Kaplan DR, Cantley LC. PI3K: downstream AKTion blocks apoptosis. Cell. 1997 Feb 21;88(4):435-7.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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