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Datasheet
Monoclonal Antibody to Phosphorylated AKT1 (Ser473)
100 ug of antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype
:
Mouse IgG1, Kappa
Clone
:
104A282
Purification
:
Protein G Chromatography
Species
:
Human, Mouse
Predicted React
:
Chicken, Frog
Host
:
Mouse
Application
IP: 1 ug/ml Western blot analysis: 2 ug/ml Storage Store at 4°C, stable for 6 months. For long-term storage, store at -20°C. Recommended Positive Control : PDGF treated NIH 3T3
Background
AKT, protein kinase B (PKB), is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activat phosphtidylinositol 3-kinase (PI 3-kinase). The major activity of AKT/PKB is to mediate cell survival. AKT/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI-3 kinase and AKT/PKB kinase activity. AKT/PKB phosphorylation is critical for it activity. The major phosphorylation sites required for AKT activation has been identified as threoinine 308 and serine 473. Serine 473 is phosphorylated by MAPKAP kinase 2.
Antigen
This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human AKT1, Accession no. ref|NP_005154.2|. The sequence in this region is identical in human, mouse, chicken, and frog.
Application Notes
Clone 104A282 specifically detects the phosphorylated form of AKT1 in PDGF treated cells.
Reference 1. Alessi DR., et al. EMBO J. 15: 6541-6551 (1996). 2. Ferring P., et al. Oncogene 18: 6129-6134 (1999). 3. Kandel ES, et al. Exp. Cell Res. 253: 210-219 (1999). 4. Cantley LC, et al. Proc Natl Acad Sci, USA, 96: 420-4245 (1999). 5. Franke TF, et al. Cell 88: 435-437 (1997).
Product Citations 1. Deguelin, an Akt Inhibitor, Suppresses IB Kinase Activation Leading to Suppression of NF-kB-Regulated Gene Expression, Potentiation of Apoptosis, and Inhibition of Cellular Invasion. Asha S. Nair, Shishir Shishodia, Kwang Seok Ahn, Ajaikumar B. Kunnumakkara, Gautam Sethi, and Bharat B. Aggarwal. J. Immunol., 177: 5612-5622 (2006). 2.
Suppression of NF-kB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes; Implications for the treatment of osteoarthritics. Shakibaei M, T John, G Schulze-Tanzil, I Lehmann, A Mobasheri. Biomedical Pharmacology 73:1434-1445. WB: primary cultures of human chondrocytes isolated from articular cartilage.
Imgenex antibodies cited for WB (primary cultures of human chondrocytes isolated from articular cartilage):
1. p65 (IMG-512), Fig 3A and B
2. IkBa (IMG-127A), Fig 3A-C
3. Phospho-IkBa (IMG-156A), Fig 3A and B
4. Phospho-Akt (IMG-187A), Fig 5
Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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