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Monoclonal Antibody to mouse TNFa |
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| Catalog No: |
IMG-225A |
| Contents: |
100 ug in 200ul PBS containing 0.09% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rat IgG1, Kappa |
| Clone: |
MP6-XT22 |
| Purification: |
Protein G Chromatography |
| Species: |
Mouse |
| Host: |
Rat |
| Application: |
ELISA: please see reference (1, 2) for details
Western blot analysis: 1-5 ug/ml |
| Storage: |
Store at 4oC, stable for 6 months. For long-term storage, store at -20oC. |
Recommended
Positive Control: |
LPS stimulated macrophages |
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Background
The purified form of clone MP6-XT3 (IMG-224A) can be used for ELISA (capture) in conjunction with biotin labeled antibody from clone MP6-XT22 (IMG-225B) (1,2), ELISPOT, and Western blotting. Use 1-5 ug/ml of antibody for western blot analysis. |
Antigen
This antibody was generated against recombinant mouse TNFa expressed in E. coli. |
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Reference
1. Abrams JS, et al. Immunol. Rev. 127:5-24 (1992).
2. Guesdon JL, et al. J. Histochem. Cytochem. 27:
1131-1139 (1979).
3. Hunter CA, Litton MJ, et al. J Infet Dis 170 (4):
939-945 (1994).
4. Litton MJ, Sander B., et al. J Immunol Methods 175
(1): 47-58 (1994).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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