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Product : Antibody >> TNF Family & Death Re.. >> DcR1
      
 

Polyclonal Antibody to DcR1 (TRAIL-R3, TRID, LIT)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-245-1
DcR1Purified
0.05 mg
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Catalog No: IMG-245-1
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.05 mg of purified antibody in PBS containing 0.02% sodium azide.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 1 ug/ml
Storage: Store at 4°C.
Recommended
Positive Control:
293 or HeLa
 

Background
Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptors have been recently identified and designated DR4 and DR5 (for death receptor 4). DR4 and DR5 are functional receptors for TRAIL, and DcR1/TRID and DcR2 are decoy receptors1-3. DcR1 is also designated as TRAIL-R3, TRID or LIT2-7. DcR1 has an extracellular TRAIL-binding domain but lacks intracellular death domain and does not induce apoptosis. TRAIL-R3 also has several sites for potential N- (5 sites) and O-linked (approximately 20) glycosylation and GPI addition. Antibodies made against TRAIL-R3/DcR1 detects a protein of approximate molecular mass of 65kDa in the western blot analysis under denaturing and reducing conditions instead of theoretical Mr of 29 kDa6. The slow migration of this protein in SDS-PAGE is probably due to the presence of 5 TAPE (threonine, alanine, proline, and glutamine-rich) repeats. The TAPE repeat-deficient receptor migrates normally in SDS-PAGE6. Unlike TRAIL-R1 (DR4) and TRAIL-R2 (DR5), DcR1/TRAIL-R3 transcript is restricted to skeletal muscle, peripheral blood lymphocytes, and the spleen.

Antigen
This antibody was raised by immunizing rabbits with a peptide corresponding to aa 149-167 at the extracellular domain of human DcR1 precursor (4-6).

Application Notes
A 65 kDa band is observed.

Genebank Info (Protein): NP_803187
Gene ID: 23405
SwissProt: Q9UPY3
 
DcR1 Antibody

Western blot analysis of 20 µg of whole cell lysate from HeLa (Lane 1) and 293 (Lane 2) cells with anti-DcR2 at 1 ug/ml dilution.

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Reference
1. Sheridan JP, Marsters SA, Pitti RM, Gurney A, Skubatch M, Baldwin D, Ramakrishnan L, Gray CL, Baker K, Wood WI, Goddard AD, Godowski P, Ashkenazi A. Science 1997; 277:818-821.
2. Pan G, O’Rourke K, Chinnaiyan AM, Gentz R, Ebner R, Ni J, Dixit VM. Science 1997;276:111-113.
3. Pan G, Ni J, Wei YF, Yu G, Gentz R, Dixit VM. Science 1997;277:815-818.
4. Degli-Esposti MA, Smolak PJ, Walczak H. et al. J Exp Med 166: 1165-1170 (1997).
5.  McFarlne M, Ahamad M, srinivasan SM et al. J. Biol. Chem 272: 25417-25420 (1997).
6. Shneider P, Bodmer JL, Thome M, Hofmann K, Holler N, and Tschopp J. FEBS Lett. 416: 329-334 (1997).
7. Mongkolsapaya J, Cowper AE, Xu XN, et al. J Immunol 160: 3-6 (1998).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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