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Product : Antibody >> Apoptosis , Cancer Antibodies , DNA Methylation & Rep.. , IHCProTM , Immunohistochemistry >> p73
      
 

Monoclonal Antibody to p73

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-246
p73Purified
0.1 mg
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Catalog No: IMG-246
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 5B429
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Mouse
Application: ChIP: See Marques-Garcia et al, 2009 for details
IF/ICC: Please see Vella et al (2003) and Sayan et al (2005) for details.
IHC (frozen): 5-15 ug/ml, see Dominguez et al, 2006 for details.
IHC (paraffin): 5-15 ug/ml, see Arfaoui et al (2010) for details.
IP: Please see Sayan et al (2005) for details.
Western blot analysis: 2 ug/ml
Storage: Store at 4°C, stable for 6 months at 4°C. For long-term storage, store at -20°C.
Recommended
Positive Control:
p73 transfected cells, HeLa cell lysate.  Please refer to Product Citations for additional examples of endogenous expression of p73.  
 

Background
p73 was identified as a long-lost cousin of tumor suppressor protein, p53 (4). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (3). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (2). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein (1). Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas it was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

Antigen
This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Application Notes
1. IMG-246 recognizes all of the TAp73 isoforms (isoforms with the intact NH2-terminal transactivation domain) (Thottassery et el, 2006). 2. The IMG-246 5B429 clone has also been referred to as 429 in the literature. 3. Endogenous expression of p73 has been detected in a variety of cell types; for example, please refer to Sayan et al (2005), Vella et al (2003) and as well as other product citations for details regarding culture and treatment conditions. 4. Immunofluorescence microscopy fixation details can be found in Vella et al (2003). 5. Immunoprecipitation details can be found in Sayan et al (2005). 6. Imgenex offers several p73 antibodies with different features, many of which have been documented through publications.  For example, Sayan et al (2005) showed that p73 IMG-246 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 IMG-259 showed reactivity against all p73 isoforms including delta Np73.  7. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described (Sayan et al, 2008). The ability of a given antibody to recognize a p73 fragment will depend on whether or not the antibody epitope is contained in the fragment. As p73 research continues to evolve, researchers are encouraged to refer to the published literature using the p73 antibodies to gain additional information.

Genebank Info (Protein): NP_005418
Gene ID: 7161
SwissProt: O15350
 
p73 Antibody WB

Western blot analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate using IMG-246 at 2 ug/ml.

p73 Antibody IHC

Formalin-fixed, paraffin-embedded breast cancer probed with p73 antibody at 5 ug/ml. IMGENEXs human tissue TMA (IMH-343) was used for this test.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Kaghad M, Bonnet H, Yang A, Creancier L, Biscan JC., et al. Cell 90: 809-819 (1997).
2. Levrero M, De Laurenzi V, Costanz0 A, Gong J, Wang JY, and Melino G. J Cell Sci 113 (pt 10): 1661-1670 (2000).
3. Yang A, Walker N, Bronson R, Kaghad M, Oosterwegel M, et al. Nature 404: 99-103 (2000).
4. Ueda Y, Hijikata M, Takagi S, Chiba T, and Shimotohno K. Oncogene 18: 4993-4998 (1999).
5. Sayan AE, BS Sayan, V Gogvadze, D Dinsdale, U Nyman, TM Hansen, B Zhivotovsky, GM Cohen, RA Knight, G Melino. p73 and caspase-cleaved p73 fragments localize to mitochondria and augment TRAIL-induced apoptosis. Oncogene 27:4363-4372 (2008). 


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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