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Product : Antibody >> TNF Family & Death Re.. >> Taj/TROY
      
 

Polyclonal Antibody to TAJ/TROY

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-254
Taj/TROYPurified
0.1 mg
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Catalog No: IMG-254
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 g in 200 l PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human, Mouse
Host: Rabbit
Application: Western blot analysis: 0.5-2 ug/ml
Storage: Store at 4C, stable for 6 months. For long-term storage, store at -20C.
Recommended
Positive Control:
T98G
 

Background
Analysis of EST clones and use of a signal sequence trap screening of the murine brain have lead to identification of a new member of the tumor necrosis factor receptor superfamily designated as TAJ and TROY, respectively (2,3). TAJ/TROY is a membrane protein of 423 amino acids with characteristic cysteine-rich motifs in the extracellular domain and a tumor necrosis factor receptor-associated factor (TRAF) 2 binding sequence in the cytoplasmic domain. Human TROY has 78% identity at the amino acid level with mouse TROY. The extracellular domain of TROY has significant homology with that of EDAR (another newly identified receptor belonging to TNF receptor family) that specifies hair follicle fate (1). The cytoplasmic domain of TAJ is different than other TNF receptor family members. Although its cytoplasmic domain does not contain death domain, TAJ/TROY induced cell death is probably mediated through a caspase-independent pathway. TAJ-induced JNK activation was not blocked by dominant-negative inhibitors of TRAF2, TRAF5, or ASK1, which have been previously implicated in JNK activation via TNFR1 and CD40. However, coimmunoprecipitation assays revealed that TAJ is capable of binding a number of different TRAF family members, and it is possible that TAJ-induced JNKactivation is mediated by an as yet untested TRAF homolog, such as TRAF6. TROY mRNA is highly expressed in brain, prostate and embryo and moderately expressed in the heart, lung, liver.

Antigen
This rabbit polyclonal antibody was developed by immunizing rabbits with a mixture of synthetic peptides corresponding to amino acids 29-44, 204-219, and 409-421 of human TROY (Genbank Accession No. AB040434).

Genebank Info (Protein): NP_683760
Gene ID: 55504
SwissProt: Q9NS68
 
Taj/TROY Antibody

Western blot analysis of TAJ/TROY using IMG-254 in T98G, a human glioblastoma cell line lysate. A protein band of approximately 46 kD is detected.

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Reference
1. Eby MT, Jasmin A, Kumar A, Sharma K, and Chaudhury P. J. Biol. Chem. 275: 15336-15342 (2000).
2. Kojima T, Morikawa Y, Copeland NG, Gilbert DJ, Jenkins nA, Senba E, and Kitamura T. J. Biol. Chem., Vol. 275: 20742-20747 (2000).
3. Monreal AW, Ferguson BM, Headon DJ, Street SL, Overbeek PA, and Zonana J. Nat. Genet. 22 (4): 366-369 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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