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Monoclonal Antibody to human TRAIL (Apo-2 Ligand) |
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| Catalog No: |
IMG-256A |
| Contents: |
100 µg antibody in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
55B709.3 |
| Purification: |
Protein G Chromatography was used to purify this TRAIL antibody |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
IHC (p): 5 ug/ml
Western blot analysis: use antibody at 1-5 ug/ml |
| Storage: |
Store TRAIL antibody at 4°C for six months. For long term storage, store antibody at -20°C. |
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Background
Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. It is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand) (6,7). TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas-L, TRAIL induces apoptosis and NF-kB activation in many tissues and cells. |
Antigen
This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 17-35 (VLIVIFTVLLQSLCVAVTY) of human TRAIL, GenBank no NP_001177872.1. The peptide sequence used for immunogen has been shown to be involved in TRAIL and DR5 (TRAIL-R2) interaction. |
Application Notes
The amino acid sequence used as immunogen for this antibody is 100% homologous in human and 95% dog and cat. |
Genebank Info (Protein)
NP_003801 |
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Western blot analysis of TRAIL in Jurkat lysate using TRAIL antibody. |
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IHC (P) analysis of TRAIL in human kidney using TRAIL antibody at 5 ug/ml. |
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Reference
1. Pan G et al. Science 276:111-113 (1997).
2. Schneider P, et al. Immunity 7:831-836 (1997).
3. Pan G, et al. Science 277:815-818 (1997).
4. Sheridan JP, et al. Science 277:818-821 (1997).
5. Mongkolsapaya, J. et al. Nat. Struct. Biol. 6: 1043 (1999).
6. Wiley SR etal. Immunity 3:673-682 (1995).
7. Pitti RM etal. J. Biol. Chem. 271:12687-90 (1996).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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