Product : Antibody >> Apoptosis , Cancer Antibodies , DNA Methylation & Rep.. , Neurobiology , Transcription Factors >> p73 (Alpha, Beta, Gamma, Delta Isoforms)

Monoclonal Antibody to Human p73(Alpha, Beta, Gamma, Delta Isoforms)

Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
p73 (Alpha, Beta, Gamma, Delta Isoforms)Purified
0.1 mg
Catalog No: IMG-259A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 5B1288
Purification: Protein G Purification
Species: Human, Mouse
Host: Mouse
Application: ChIP: see Accardi et al (2006) for details
IF/ICC: Please see Sayan et al (2005) for details.
IP: Please see Sayan et al (2005) for details.
Western blot analysis: 1-3 ug/ml
Storage: Aliquot and store at -20C.
Positive Control:
p73 transfected cells. Please refer to Product Citations for examples of endogenous expression of p73.

p73 was identified as a long-lost cousin of tumor suppressor protein, p53 (4). p73 has high homology with p53 as well as with p63, a gene implicated in the maintenance of epithelial stem cells. Significant homology between p53, p63, and p73 (approximately 63% amino acid identity in the DNA-binding domain suggest that they may have overlapping functions in the regulation of gene expression (3). The targeted disruption of p73 gene leads to defects hippocampal dysgenesis, hydrocephalus, chronic inflammation and infections (2). Recently, spilicing variant mRNAs of p73 has been identified in MCF-7, a breast carcinoma cell line. These mRNAs code for variant p73 proteins bearing distinct carboxy-terminal structures suggesting that the carboxy-terminal region of p73 may be important for the functions of this protein (1). Tumor BioMarker: Vella et al (2003) found p73 to be upregulated in a significant fraction of anaplastic thyroid cancers, whereas p73 was not detectable in normal thyroid epithelial cells nor in papillary or follicular thyroid cancer.

This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule. It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. The antibody does not cross react with p53.

Application Notes
1. The clone has also been referred to as 1288 in the literature. 2. Endogenous expression of p73 has been detected in a variety of cell types; please refer to Product Citations for details regarding culture and treatment conditions. 3. Immunoprecipitation details can be found in Sayan et al (2005). 4. IMG-259A has been shown to recongize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). 5. Imgenex offers several p73 antibodies with different features, many of which have been documented through publications. For example, Sayan et al (2005) showed that p73 IMG-246 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 IMG-259 showed reactivity against all p73 isoforms including delta Np73. Endogenous expression of p73 has been detected in a variety of cell types; please refer to Product Citations for details regarding culture and treatment conditions. Immunoprecipitation details can be found in Sayan et al (2005). 6. 7. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described (Sayan et al, 2008). The ability of a given antibody to recognize a p73 fragment will depend on whether or not the antibody epitope is contained in the fragment. As p73 research continues to evolve, researchers are encouraged to refer to the published literature using the p73 antibodies to gain additional information.

Genebank Info (Protein): NP_005418
Gene ID: 7161
SwissProt: O15350
p73 (Alpha, Beta, Gamma, Delta Isoforms) Antibody

Western blot analysis of p73 in transfected cell lysate using IMG-259A at1 ug/ml.

p73 (Alpha, Beta, Gamma, Delta Isoforms) Antibody IMG-259A

Western blot analysis of p73 in HeLa cell lysate (Cat no. 40161) using IMG-259A at1 ug/ml.


1. Kaghad M, Bonnet H, Yang A, Creancier L, Biscan JC., et al. Cell 90: 809-819 (1997).
2. Levrero M, De Laurenzi V, CostanzO A, Gong J, Wang JY, and Melino G. J Cell Sci 113 (pt 10): 1661-1670 (2000).
3. Yang A, Walker N, Bronson R, Kaghad M, Oosterwegel M, et al. Nature 404: 99-103 (2000).
4. Ueda Y, Hijikata M, Takagi S, Chiba T, and Shimotohno K. Oncogene 18: 4993-4998 (1999).
5. Sayan AE, BS Sayan, V Gogvadze, D Dinsdale, U Nyman, TM Hansen, B Zhivotovsky, GM Cohen, RA Knight, G Melino. p73 and caspase-cleaved p73 fragments localize to mitochondria and augment TRAIL-induced apoptosis. Oncogene 27:4363-4372 (2008).

Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.






  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways