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Monoclonal Antibody to Dnmt1 (DNA methyltransferase 1) |
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| Catalog No: |
IMG-261A |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
60B1220.1 |
| Purification: |
Protein G Chromatography was used to purify the antibody |
| Species: |
Human, Mouse, Zebrafish |
| Host: |
Mouse |
| Application: |
ChIP: please see product citation Yu-Wei Leu et al for details
IHC (paraffin): use antibody at 1-2 ug/ml
IP: see product citations listing IP for details.
Western blot analysis: 0.1-0.5 ug/ml
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| Storage: |
Store antibody at 4șC. For long-term storage, store at -20șC. |
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
IMG-261A recognizes Dnmt1, a 1616 amino acid protein according to GenBank no. NP_001370. |
Antigen
This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370). It will cross react with mouse Dnmt1. |
| Genebank Info (Protein): NP_001124295 |
| Gene ID: 1786 |
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Western blot analysis of Dnmt1 in 2102EP (human embryonic carcinoma) in the A) absence and B) presence of immunizing peptide, recombinant human Dnmt1 protein in the C) absence and D) presence of immunizing peptide, NIH 3T3 (mouse embryonic fribroblast) in the E) absence and F) presence of immunizing peptide, and D3 (mouse embryonic stem cell) in the G) absence and H) presence of immunizing peptide using Dnmt1 antibody. IMG-261A was used at 0.5 ug/ml for testing the human cell line and 0.1 ug/ml was used for testing the recombinant protein and the mouse cell lines. IMGENEX’s goat anti-mouse Ig HRP secondary antibody (IMGENEX, 20101) and PicoTect ECL substrate solution (IMGENEX, 10087K) were used for this test. |
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IHC analysis of Dnmt1 using antibody at 2 ug/ml on formalin-fixed, paraffin-embedded medullar kidney tissue sections |
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Reference
1. Bestor. T., et al.. J.Mol. Biol. 203: 971-983 (1988).
2. Yen RW, Vertino PM, Nelkin BD et al. Nucl. Acids Res. 20: 2287-2291 (1992).
3. Xie, S. et al. Gene 236: 87-95 (1999).
4. Okano M, Bell DW, Haber DA,a nd Li E. Cell 99: 247-257 (1999).
5. Reik W. et al. J. Nat. Genet 23: 380-382 (1999).
6. Robertson KD, et al. Nat Genet 25(3):338-342 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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