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Product : Antibody >> Chromatin , DNA Methylation & Rep.. , IHCProTM , Immunohistochemistry >> Dnmt3a
      
 

Monoclonal Antibody to Dnmt3a (DNA methyltransferase 3a)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-268A
Dnmt3aPurified
0.1 mg
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Catalog No: IMG-268A
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg antibody in 0.2ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Mouse IgG1, Kappa
Clone: 64B1446
Purification: Protein G Chromatography was used to purify the antibody.
Species: Human, Mouse
Host: Mouse
Application: ChIP: Please see Product Citations below
IF/ICC: 5 ug/ml
IHC (paraffin): use antibody at 5 ug/ml
Western blot analysis: 2 ug/ml
Storage: Store antibody at 4ºC. For long-term storage, aliquot antibody and store at -20ºC.
 

Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Antigen
This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details.  

Genebank Info (Protein): NP_715640
Gene ID: 1788
SwissProt: Q9Y6K1

Genebank Info (Nucleotide): AF068625

 
Dnmt3a Antibody WB

Western blot analysis of (A) Dnmt3a transfected 293 cell lysate (IMGENEX,  40212) and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.

Dnmt3a Antibody

Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. (Data courtsey of A. Jane Roskams, University of British Columbia).

Dnmt3a Antibody IHC

Formalin-fixed, paraffin-embedded human liver (IMH-343) stained with Dnmt3a antibody at 5 ug/ml.

Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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Reference
1. Xie S, Wang Z, Okano M, Nogami M, Li Y, He WW, Okumura K, and Li E. Gene 236: 87-95 (1999).
2. Okano M, Bell DW, Haber DA, and Li E. Cell 99: 247-257 (1999).
3. Reik W, Kelsey G, and Walter J. Nat. Genet 23: 380-382 (1999).
4. Hsieh CL. Mol Cell Biol 19: 8211-8218 (1999).
5. Hansen RS, Wijmenga C, Juo P., Stanek AM, canfield TK, Weemaes CM, and Gartler SM. Proc. Natl. Acad. Sci USA 96: 14412-14417 (1999).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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