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Monoclonal Antibody to Dnmt3a (DNA methyltransferase 3a) |
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| Catalog No: |
IMG-268A |
| Contents: |
0.1 mg in 0.2ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
64B1446 |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Mouse |
| Application: |
ChIP: Please see Product Citations below
IF/ICC: 5 ug/ml
IHC (paraffin): Please see Product Citations below
Western blot analysis: 2 ug/ml |
| Storage: |
Store at 4ºC. For long-term storage, store at -20ºC. |
Recommended
Positive Control: |
transfected cell lysate (WB), HeLa cells (ICC) |
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
Antigen
This antibody was raised against bacteria expressed recombinant mouse Dnmt3a. The epitope was found to lie near the C-terminus (a.a. 705-908), see Chen et (2002) for details. |
| Genebank Info (Protein): NP_715640 |
| Gene ID: 1788 |
| SwissProt: Q9Y6K1 |
Genebank Info (Nucleotide): AF068625 |
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Expression of Dnmt3a in the nuclei of post-mitotic neurons in the olfactory epithelium (OE). DNMT3a (red) is co-expressed with neuron-specific tubulin (green) throughout the development of the olfactory epithelium. (Data courtsey of A. Jane Roskams, University of British Columbia). |
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Western blot analysis of Dnmt3a in (A) Dnmt3a transfected 293 cell lysate (IMGENEX, 40212) and (B) untransfected 293 cell lysate using IMG-268A at 1 ug/ml. |
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Reference
1. Xie S, Wang Z, Okano M, Nogami M, Li Y, He WW, Okumura K, and Li E. Gene 236: 87-95 (1999).
2. Okano M, Bell DW, Haber DA, and Li E. Cell 99: 247-257 (1999).
3. Reik W, Kelsey G, and Walter J. Nat. Genet 23: 380-382 (1999).
4. Hsieh CL. Mol Cell Biol 19: 8211-8218 (1999).
5. Hansen RS, Wijmenga C, Juo P., Stanek AM, canfield TK, Weemaes CM, and Gartler SM. Proc. Natl. Acad. Sci USA 96: 14412-14417 (1999).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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