PATHWAY PROFILING ANTIBODIES & REAGENTS

     
Products
Product : Antibody >> Apoptosis , Cancer Antibodies , Cytokine & Chemokine , Signal Transduction , TNF Family & Death Re.. >> STAT1a/b
      
 

Polyclonal Antibody to STAT1a/b

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-280
STAT1a/b Purified
0.1 mg
 --
 
Catalog No: IMG-280
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 ug in 200 ul PBS containing 0.2% gelatin, 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Purification: Protein G Chromatography
Species: Chimpanzee, Dog, Human, Mouse, Pig
Predicted React: Chicken, Rat
Host: Rabbit
Application: Western blot analysis: 1-2 ug/ml
Storage: Store at 4C for six months. For long term storage, store at -20C.
Recommended
Positive Control:
HeLa
 

Background
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. STAT1 is involved in the TNFR1-TRADD signaling complex. In Hela cells STAT1 acts as a TNFR1-signaling molecule to suppress NF-kB activation. It has also been shown that its activation slows growth and promotes apoptosis.

Antigen
A synthetic peptide sequence corresponding to amino acids 711-730 EVHPSRLQTTDNLLPMSPEE of human Stat1 was used as immunogen: GenBanK no.NP_009330.1. This sequence is 100% conserved between Stat1 alpha and Stat1 beta.

Application Notes
This antibody is able to detect both the alpha form (p91, ~91 kD) and the beta form (p84, ~84 kD) of STAT1. This is because the amino acid sequence used as immunogen is 100% homologous in both forms. The amino acid sequence used is 100% homologous in human, mouse, dog, pig and chimp, 86% in rat and 80% in chicken.

Genebank Info (Nucleotide): NP_009330.1.

 
STAT1a/b  Antibody

Western blot analysis of STAT1 in HeLa cell lysate using IMG-280 at 1 ug/ml.

-

Reference
1. Jacqueline M., et al. J.Biol. Chem. 273:31408-31416(1998)
2. Anastasis Stephanou, et al. J. Biol. Chem 275 (14) :10002-10008 (2000)
3. Wang Y, et al. Mol Cell Biol Jul;20(13):4505-12(2000)


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


Products

Services

Resources

Purchasing

Company

  • What's New
  • Reporter Cells
  • Screening Services
  • Interactive Pathways