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Polyclonal Antibody to Dnmt2 (DNA methyltransferase 2) |
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| Catalog No: |
IMG-281 |
| Contents: |
100 ug antibody in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: use antibody at 2-4 ug/ml
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| Storage: |
Store Dnmt2 antibody at 4°C for six months. For long term storage, store antibody at -20°C. |
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Background
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases. |
Antigen
This antibody was raised against synthetic peptides corresponding amino acids 39-54 (ANEVYKHNFPHTHLLS) and 361-376 (PEKTTVKQRYRLLGNS) of mouse Dnmt2, GenBank no. ref|NP_034197.3| . |
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Western blot analysis of Dnmt2 in mouse ES cell lysate using Dnmt2 antibody at 2 ug/ml. |
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Reference
1. Yoder JA, Bestor TH. Hum Mol Genet 7(2):279-84 (1998).
2. Okano M, Xie S, Li E.: Nucleic Acids Res 26:2536-40 (1998).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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