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Polyclonal Antibody to ACT1 (NF-kB activator 1) |
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| Catalog No: |
IMG-284 |
| Contents: |
100 µg in 200 µl PBS containing 0.02% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit Ig |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store at 4°C, stable for 6 months. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
MCF-7 |
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Background
This product has been discontinued. Please see IMG-563.
NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes. |
Antigen
A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574 (PRGPLPTLQVVPLC) of human ACT1 was used as immunogen, GenBank no. gi|10242290|gb|AAG15367.1|AF274303_1 . |
Application Notes
In MCF-7, a ~60 kDa band is observed. |
Genebank Info (Protein)
NP_679211 |
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Western blot analysis of 10 µg of total cell lysate from MCF-7, a human breast cancer cell line, with IMG-284 at 1 ug/ml dilution. |
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Reference
1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).
3. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, and Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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