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Product : Antibody >> NF-kB Pathway , Th17 Antibodies >> ACT1/CIKS - discontinued, please see IMG-563
      
 

Polyclonal Antibody to ACT1 (NF-kB activator 1)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-284
ACT1/CIKS - discontinued, please see <a href="http://www.imgenex.com/search_result.php?txtSearch=IMG-563">IMG-563</a>Purified
0.1 mg
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Catalog No: IMG-284
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 100 g in 200 l PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit Ig
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Host: Rabbit
Application: Western blot analysis: 1-2 ug/ml
Storage: Store at 4C, stable for 6 months. For long-term storage, store at -20C.
Recommended
Positive Control:
MCF-7
 

Background

This product has been discontinued.&nbsp; Please see IMG-563.

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (3). External stimuli such as tumor necrosis factor or other cytokines, such as IL-1 results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes (2). Recently, Li et al. (1) have isolated a cDNA coding for a protein named Act1 (NF- B activator 1). Act1 cDNA codes for a protein containing 574 aa with a predicated molecular mass of 60 kDa. Act1 protein contains a helix-loop-helix domain in its N-terminal portion and a coiled-coil structure at the C terminus. Act1 lacks any catalytic domain, however, it activates IKK through the helix-loop-helix domain. The activation of NF- B by Act1 appears to be through the NIK-IKK complex, because a NIK dominant-negative mutant protein inhibited Act1-induced NF- B activation. IL-1 responsive pathway is also necessary for Act1 function. Act1 mRNA is expressed in a variety of tissues, with high level in thymus, kidney and placenta; moderate level in heart, skeletal muscle, colon, liver, lung and small intestine; and at a very low level in brain, spleen, and peripheral blood leukocytes.

Antigen
A mixture of synthetic peptides corresponding to amino acids 341-351 (CSFPGLPRHQDQPHHQ), 91-104 (CLRTQVLEDSEDSF) and 562-574(PRGPLPTLQVVPLC)of human ACT1 was used as immunogen, GenBank no. gi|10242290|gb|AAG15367.1|AF274303_1.

Application Notes
In MCF-7, a ~60 kDa band is observed.

Genebank Info (Protein): NP_679211
Gene ID: 10758
 
ACT1/CIKS - discontinued, please see IMG-563 Antibody

Western blot analysis of 10 g of total cell lysate from MCF-7, a human breast cancer cell line, with IMG-284 at 1 ug/ml dilution.

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Reference
1. Verma, I.M., Stevenson, J.K., Schwarz, EM., Van Antwerp, D. & Miyamoto, S. Genes. Dev. 9: 2723-2735 (1995).
2. Verma, I. And Stevenson, J.K. Proc. Nat. Acad. Sci. USA 94: 11758 (1997).
3. Li X, Commane M, Nie H, Hua X, Chatterjee-Kishore M, Wald D, Haag M, and Stark GR. Proc Natl Acad Sci U S A 97(19):10489-10493 (2000).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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