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Polyclonal Antibody to Human Rad1 (hRad1) |
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| Catalog No: |
IMG-288 |
| Contents: |
100 ug in 200 ul PBS containing 0.02% sodium azide. Sodium azide is highly toxic |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human, Mouse |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store at 4°C, stable at 4°C for 6 months. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
293, HeLa, or NIH 3T3 |
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Background
The structure and integrity of DNA molecules can be spontaneously affected through intrinsic instability of chemical bonds in DNA or can be induced by chemical compounds and irradiation. DNA lesions are of many different types including single- and double-strand breaks, inter- and intra-strand cross-links and different kinds of base modification (3,4). DNA lesions hamper processes like transcription and replication resulting in cell cycle arrest, apoptosis and genomic instability (mutagenesis). DNA lesions have been implicated in many distinct genetically inherited diseases, in carcinogenesis, origin of genetic disorders and in aging (2-4). Rad1 is an essential component of cell cycle checkpoints activated by DNA damage and incomplete DNA replication (1). Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage checkpoint response in S. pombe. Human HRAD1 gene is located on chromosome 5p13. |
Antigen
The antibody was raised against a synthetic peptide corresponding to the amino acid 133-148 of human Rad1, which is a homolog of Schizosaccharomyces pombe Rad1. It will cross-react with mouse Rad1. |
Application Notes
A ~66 kDa band is observed. |
Genebank Info (Protein)
NP_596868 |
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The anti-Rad1 rabbit PcAb (Cat#IMG-288) was diluted at 2 µg/ml (lane 1) and 1 ug/ml (lane 2) and tested against 10 ug of 293 cell lysate by western blotting. A single band at 66 kDa is detected. |
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Reference
1. Schar P. Cell. 2001, 104:329-32.
2. van Zeeland AA, Mullenders LH, Vrieling H. Mutat Res. 2001, 485:15-21.
3. Rich T, Allen RL, Wyllie AH. Nature. 2000, 407:777-83.
4. Araujo SJ and Wood RD. Mutat Res. 1999, 435:23-33.
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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