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Polyclonal Antibodies to STAT5b |
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| Catalog No: |
IMG-292 |
| Contents: |
0.1 mg in 0.2 mll PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Rabbit IgG |
| Clone: |
N/A |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Predicted React: |
Bovine, Chicken, Mouse, New World monkey, Rat |
| Host: |
Rabbit |
| Application: |
Western blot analysis: 1-2 ug/ml |
| Storage: |
Store at 4șC, stable at 4șC for 6 months. For long-term storage, store at -20șC. |
Recommended
Positive Control: |
Jurkat |
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Background
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2). Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion (3, 4). Stat5b may also act as a transcriptional inhibitor as demonstrated by inhibition of NF-kB mediated signaling (5). This Stat5b-mediated inhibitory effect on NF-kB signaling does not depend on Stat5b-DNA interactions but requires the carboxyl terminus of Stat5b as well as Stat5b nuclear translocation and/or accumulation, suggesting that Stat5b is competing for a nuclear factor(s) necessary for NF-kB-mediated activation of target promoters. |
Antigen
This polyclonal antibody was developed against a synthetic peptide corresponding to amino acids 771-787 of human Stat5b. This peptide is identical to homologues amino acid sequence in mouse, rat, bovine, monkey and chicken. |
Application Notes
In Jurkat, a 92 kDa band is observed. |
| Genebank Info (Protein): NP_036580 |
| Gene ID: 6777 |
| SwissProt: P51692 |
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Western blot analysis of Stat5b in 10 ug of Jurkat cell lysate using 1 ug/ml IMG-292. Immunoreaction was detected by chemiluminescence |
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Reference
1. Luo G and Yu-Lee L. Stat5b inhibits NF-kB mediated
signaling. Mol Endocrinol 14: 114-123 (2000).
2. Leonard WJ, O'Shea JJ. Jaks and STATs: biological
implications. Annu Rev Immunol 16:293-322 (1998).
3. Schindler C, Darnell JE Jr. Transcriptional responses to
polypeptide ligands: the JAK-STAT pathway. Annu Rev
Biochem 64:621-51 (1995).
4. Darnell JE Jr. STATs and gene regulation. Science
277:1630-1635 (1997).
5. Sexl V, Piekorz R, Moriggl R, Roherer J, et al. Stat5a/b
contribute to interleukin 7-induced B-cell precursor
expansion, but bcr/abl-induced transformation are
independent of stat5. Blood 96: 2277-2283 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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