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Product : Antibody >> Apoptosis , Cancer Antibodies , Cytokine & Chemokine , Signal Transduction , TNF Family & Death Re.. >> STAT5b
      
 

Polyclonal Antibodies to STAT5b

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-292
STAT5bPurified
0.1 mg
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Catalog No: IMG-292
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg in 0.2 mll PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Isotype: Rabbit IgG
Clone: N/A
Purification: Protein G Chromatography
Species: Human
Predicted React: Bovine, Chicken, Mouse, New World monkey, Rat
Host: Rabbit
Application: Western blot analysis: 1-2 ug/ml
Storage: Store at 4C, stable at 4C for 6 months. For long-term storage, store at -20C.
Recommended
Positive Control:
Jurkat
 

Background
STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile (1,2). Both Stat5a and Stat5b regulate interleukin-7 induced B-cell precursor expansion (3, 4). Stat5b may also act as a transcriptional inhibitor as demonstrated by inhibition of NF-kB mediated signaling (5). This Stat5b-mediated inhibitory effect on NF-kB signaling does not depend on Stat5b-DNA interactions but requires the carboxyl terminus of Stat5b as well as Stat5b nuclear translocation and/or accumulation, suggesting that Stat5b is competing for a nuclear factor(s) necessary for NF-kB-mediated activation of target promoters.

Antigen
This polyclonal antibody was developed against a synthetic peptide corresponding to amino acids 771-787 of human Stat5b. This peptide is identical to homologues amino acid sequence in mouse, rat, bovine, monkey and chicken.

Application Notes
In Jurkat, a 92 kDa band is observed.

Genebank Info (Protein): NP_036580
Gene ID: 6777
SwissProt: P51692
 
STAT5b Antibody

Western blot analysis of Stat5b in 10 ug of Jurkat cell lysate using 1 ug/ml IMG-292. Immunoreaction was detected by chemiluminescence

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Reference
1. Luo G and Yu-Lee L. Stat5b inhibits NF-kB mediated signaling. Mol Endocrinol 14: 114-123 (2000).
2. Leonard WJ, O'Shea JJ. Jaks and STATs: biological implications. Annu Rev Immunol 16:293-322 (1998).
3. Schindler C, Darnell JE Jr. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway. Annu Rev Biochem 64:621-51 (1995).
4. Darnell JE Jr. STATs and gene regulation. Science 277:1630-1635 (1997).
5. Sexl V, Piekorz R, Moriggl R, Roherer J, et al. Stat5a/b contribute to interleukin 7-induced B-cell precursor expansion, but bcr/abl-induced transformation are independent of stat5. Blood 96: 2277-2283 (2000).


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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