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Peptide-affinity Purified Polyclonal Antibody to GPX4 |
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| Catalog No: |
IMG-30126 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Host: |
Goat |
| Application: |
IHC (paraffin): 0.3µg/ml
Western blot analysis: 0.1-0.3µg/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Kidney and Testis lysates |
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Background
Principal Names: GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase) ; HGNC:4556; PHGPx; snGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase Official Symbol: GPX4 Accession Number(s): NP_002076.2; NP_001034937.1 Human GeneID(s): 2879 Important Comments: This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1). |
Antigen
Peptide with sequence CEEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2; NP_001034937.1. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3µg/ml.Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3µg/ml. |
Genebank Info (Protein)
NP_001034937 |
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Western Blot analysis of GPX4 in Human Testis lysate using IMG-30126. |
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IHC analysis of GPX4 in paraffin embedded Human Testis using IMG-30126 at 0.3µg/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. |
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Reference
1. Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN.
Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation.
J Biol Chem. 2005 May 18; [Epub ahead of print]
PMID: 15901730
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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