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Product : Antibody >> DNA Methylation & Rep.. , Metabolism >> GPX4
      
 

Peptide-affinity Purified Polyclonal Antibody to GPX4

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-30126
GPX4Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-30126
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Goat
Application: IHC (paraffin): 0.3g/ml
Western blot analysis: 0.1-0.3g/ml
Storage: Store at -20C.
Recommended
Positive Control:
Human Kidney and Testis lysates
 

Background
Principal Names: GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase) ; HGNC:4556; PHGPx; snGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; sperm nucleus glutathione peroxidase Official Symbol: GPX4 Accession Number(s): NP_002076.2; NP_001034937.1 Human GeneID(s): 2879 Important Comments: This antibody is expected to recognize isoform A (NP_002076.2) and isoform C (NP_001034937.1).

Antigen
Peptide with sequence CEEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2; NP_001034937.1.

Application Notes
Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration: 0.1-0.3g/ml.Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3g/ml.

Genebank Info (Protein): NP_001034937
Gene ID: 2879
SwissProt: P36969
 
GPX4 Antibody

Western Blot analysis of GPX4 in Human Testis lysate using IMG-30126.

GPX4 Antibody IMG-30126

IHC analysis of GPX4 in paraffin embedded Human Testis using IMG-30126 at 0.3g/ml. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

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Reference
1. Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. Mammalian selenoprotein thioredoxin/glutathione reductase: Roles in disulfide bond formation and sperm maturation. J Biol Chem. 2005 May 18; [Epub ahead of print] PMID: 15901730


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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