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Peptide-affinity Purified Polyclonal Antibody to ATF4 |
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| Catalog No: |
IMG-3027 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human, Mouse |
| Predicted React: |
Mouse, Rat |
| Host: |
Goat |
| Application: |
Western blot analysis: 1-3 ug/ml |
| Storage: |
Store antibody at -20°C. |
Recommended
Positive Control: |
Raji |
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Background
Principal Names – ATF4; ATF4 Antibody; CREB2; TXREB; CREB-2; TAXREB67; activating transcription factor 4 (tax-responsive enhancer element B67) Official Gene Symbol - ATF4 GenBank Accession Number –NP_001666 LocusLink ID - 468 (human); 11911 (mouse); 79255 (rat) Gene Ontology terms - RNA polymerase II transcription factor activity; DNA binding; regulation of transcription, DNA-dependent; nucleus |
Antigen
Peptide with sequence EEVRKARGKKRVP, from C Terminus of the protein sequence according to NP_001666. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western blot: Approx 40kDa band seen in Raji cell lysate. Recommended for use at 1-3 µg/ml. An anonymous customer found positive results in WB on Mouse embryonic fibroblasts (3T3 cells). |
Genebank Info (Protein)
NP_001666 |
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ATF4 Antibody staining (1 µg/ml) of Raji lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. ATF4 antibody detected by western blot using chemiluminescence. |
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Reference
1. Tsujimoto A, Nyunoya H, Morita T, Sato T, Shimotohno K. Isolation of cDNAs for DNA-binding proteins which specifically bind to a tax-responsive enhancer element in the long terminal repeat of human T-cell leukemia virus type I. J Virol 1991 Mar;65(3):1420-6 PMID: 1847461
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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