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Monoclonal Antibody to human TLR6 (Toll-like receptor 6)/CD286 |
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| Catalog No: |
IMG-304A |
| Contents: |
0.1 mg in 0.2 ml PBS containing 0.05% BSA, 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1 |
| Clone: |
86B1153.2 |
| Purification: |
Protein G Chromatography |
| Species: |
Human |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): 0.5-1 ug/10^6 cells
IHC (paraffin): 5 ug/ml |
| Storage: |
Store antibody at 4°C. For long-term storage, store at -20°C. |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described. The amino acid sequence of hTLR6 is most similar to hTLR1 with 69% identity at the amino acid level. Human TLR6 consists of a predicted 807 amino acids with a molecular weight of approximately 92 kDa (3). Human and mouse TLR6 share an amino acid identity of 73%. TLR6 activates both NF-kB and c-Jun N-terminal Kinase (JNK). The amino acids in the cytoplasmic domain of IL-1 receptor, which are critical for NF-kB activation, are conserved in TLR6. It has also been shown that TLR2-mediated response to a phenol-soluble factor from staphylococcus epidermidis is enhanced by TLR6 (4). TLR6 is predominantly expressed in spleen, thymus, ovary, and lung (3). |
Antigen
This antibody was developed against a synthetic peptide corresponding to amino acids 408-424 of human TLR6. |
Genebank Info (Protein)
NP_006059 |
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Cell surface flow cytometric analysis of TLR6 antibody in 10^6 human monocytes using 0.5 ug of IMG-304A. Shaded histogram represents cells without antibody; green represents isotype control antibody (IMGENEX, 20109); red represents anti-TLR6 antibody. IMGENEX's goat anti-mouse IgG PE conjugated secondary antibody (IMGENEX, 20103 ) was used. |
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Immunohistochemical analysis of TLR6 in formalin-fixed, paraffin-embedded liver tissue using an isotype control antibody (top) and IMG-304A (bottom) at 5 ug/ml. |
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Reference
1. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999).
2. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
3. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
4. Hajjar AM, O’Mahony DS, Ozinsky A, Underhill DM, Aderem A, Klebanoff SJ, and Wilson CB. J Immunol. 166:15-19 (2001).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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