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Monoclonal Antibody to TLR9/CD289 (Clone 26C593.2) |
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| Catalog No: |
IMG-305A |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
26C593.2 |
| Purification: |
Protein G Chromatography |
| Species: |
Dog, Horse, Human, Mouse, Rat, Rhesus Monkey |
| Host: |
Mouse |
| Application: |
Confocal Microscopy: see Greene (2005)
EMSA: see Kindrachuk (2008).
Flow (Cell Surface): see Lee (2006)
Flow (Intracellular): 0.1-2 ug/ 1x10^6 cells
IF/ICC: see Greene et. al. (2005), see Tabeta
IHC (frozen): 10-20 ug/ml see Miller (2005).
IHC (paraffin):see Martin-Armas (2005)
IP: See Zuo et al (2012) for details.
Western blot analysis: 1-5 ug/ml
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| Storage: |
Store antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. |
Recommended
Positive Control: |
Human PBMCs, Ramos cells, Intestine, Testis |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (5) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. |
Antigen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). |
Application Notes
In human PBMC, a ~120 kDa band is observed. A smaller isoform, TLR9 isoform B (Genbank accession no. AAF72190) containing 975 amino acids may also be observed in some cases.
TLR9 Cleavage: Proteolytic cleavage is thought to occur in the endolysosome and be required for TLR9 activation. The cleavage results in different size fragments depending on the protease. Approximately 45 kD, 65 kD and 80 kD cleavage fragments have been observed (Ewald, et al, 2008 and Park, et al, 2008-please see Reference section). |
| Genebank Info (Protein): NP_059138 |
| Gene ID: 54106 |
| SwissProt: Q9NR96 |
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Western blot analysis of TLR9 antibody in A) human PBMCs, B) human intestine, C) mouse intestine and D) rat intestine tissue lysates using IMG-305A at 3 µg/ml and IMGENEXs goat anti-mouse HRP conjugate antibody (IMGENEX, 20101) as secondary. |
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Intracellular flow analysis of TLR9 in human PBMC using 0.5 ug of TLR9 antibody (red) and isotype control antibody (green). |
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IHC analysis of TLR9 in Adenocarcinoma of the lung (A), A549 cells (C), and malignant lung tissues (F) at 1:100 dilution, (600 × magnification). |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001)
4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999).
5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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