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Monoclonal Antibody to TLR9/CD289 (Clone 26C593.2) Alexa FluorŽ 488 Conjugate |
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| Catalog No: |
IMG-305AF488 |
| Contents: |
100 ug in 200 ul PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
26C593.2 |
| Purification: |
Protein G Chromatography |
| Species: |
Dog, Horse, Human, Mouse, Rat, Rhesus Monkey |
| Host: |
Mouse |
| Application: |
Confocal Microscopy: see Greene et. al. (2005)
Flow (Cell Surface): see Lee et al. (2006)
Flow (Intracellular): 0.5-1 ug/10^6 cells
IF/ICC: see Greene et. al. (2005) |
| Storage: |
Store at 4°C, stable for 6 months. DO NOT FREEZE; MATERIAL IS LIGHT-SENSITIVE. |
Recommended
Positive Control: |
Ramos cells |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (5) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. |
Antigen
This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). |
| Genebank Info (Protein): NP_059138 |
| Gene ID: 54106 |
| SwissProt: Q9NR96 |
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Intracellular flow analysis of TLR9 in Ramos cells using 0.5 ug of IMG-305AF488. Green represents isotype control (IMGENEX, 20108); red represents anti-TLR9 antibody. IMGENEX's TLR intracellular flow kit (IMGENEX, 10098K) was used for this test. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001)
4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999).
5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
Alexa FluorŽ is a registered trademark of Molecular Probes, Inc. IMGENEX sells Alexa FluorŽ dye conjugated antibodies under license from Molecular Probes, Inc. for research use only.
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