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Monoclonal Antibody to TLR9/CD289 (Clone 26C593.2) |
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| Catalog No: |
IMG-305D |
| Contents: |
0.1 mg antibody in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
26C593.2 |
| Purification: |
Protein G Chromatography |
| Species: |
Dog, Horse, Human, Mouse, Rat, Rhesus Monkey |
| Host: |
Mouse |
| Application: |
Confocal Microscopy: see Greene et. al. (2005)
FC (Intracellular): 0.1-0.5 ug/1x10^6 cells
Flow (Cell Surface): see Lee et al. (2006)
IHC (frozen): 10-20 ug/ml (see Miller et al, 2005).
IHC (paraffin): see Martin-Armas et al. (2005) |
| Storage: |
Product is light- and temperature-sensitive. Store in the dark at 4°C. Do not freeze. |
Recommended
Positive Control: |
Ramos, human PBMCs |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). By using a BLAST search, Hemmi et al., 2000 (4) have identified and subsequently isolated a cDNA coding for TLR9. Gene knockout experiments suggest that TLR9 acts as a receptor for unmethylated CpG dinucleotides in the bacterial DNA (4). Human and mouse TLR9 share an overall amino-acid identity of 75.5%. TLR9 is highly expressed in spleen. |
Antigen
This antibody was developed against a KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9. |
Genebank Info (Protein)
NP_059138 |
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Intracellular flow analysis of TLR9 in human PBMCs using 0.2 ug of IMG-305D. Shaded histogram represents cells without antibody; green represents a mouse IgG1-PE isotype control (BD Biosciences, Cat no. 554680); red represents anti-TLR9 antibody. |
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Intracellular flow analysis of TLR9 in Ramos cells using 0.1 ug of IMG-305D. Shaded histogram represents Ramos cells without antibody; green represents a mouse IgG1-PE isotype control (BD Biosciences, Cat no. 554680); red represents anti-TLR9 antibody. |
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Reference
1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2):157-161 (2001)
4. Takeuchi O, Kawai T, Sanjo H, Copeland NG, Gilbert DJ, Jenkins NA, Takeda K, and Akira S. Gene 231: 59-65 (1999).
5. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Shizo A. Nature 408: 740-745 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for six months from the date of purchase.
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