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Peptide-affinity Purified Polyclonal Antibody to GAPDH - Loading Control |
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| Catalog No: |
IMG-3073 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Cow, Dog, Drosophila, Human, Mouse, New World monkey, Pig, Rat |
| Predicted React: |
Xenopus |
| Host: |
Goat |
| Application: |
ELISA: >1:16000
IHC (paraffin): 0.3µg/ml
Western blot analysis: 0.01-0.1 ug/ml |
| Storage: |
Aliquot and store antibody at -20°C. |
Recommended
Positive Control: |
Most cell lines and tissues will be positive for GAPDH. |
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Background
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate.
The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a “housekeeping” gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization. |
Antigen
Peptide with sequence HQVVSSDFNSDT, from C Terminus of the GAPDH protein sequence according to NP_002037 was used as immunogen for this antibody. |
Application Notes
A GAPDH antibody is a widely used loading control for quantitative Western blotting. It is highly conserved across species and antibodies to GAPDH typically have broad species reactivity. |
Genebank Info (Protein)
NP_002037 |
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IHC staining of paraffin embedded Human Tonsil using GAPDH antibody at 0.3µg/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal center cells. B) Staining of endothelial cells. |
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Application of the GAPDH antibody as a loading control in Western blots. Total proteins from various mouse tissue lysates were normalized using the GAPDH polyclonal antibody at 0.05 ug/ml. Cat nos: Brain (40101), Heart (40102), Kidney (40104), Liver (40105), Lung (40106), Stomach (40110), Spleen (40109), Ovary (40108), and Testis (40111). |
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Application of the GAPDH antibody as a loading control in Western blots. Total proteins from various human tissue lysates were normalized using the GAPDH polyclonal antibody at 0.05 ug/ml. Cat nos: Kidney (40144), Liver (40145), Stomach (40152), and Testis (40150). |
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Reference
1. Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM,Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med. 1996 Mar;2(3):347-50. PMID: 8612237
2. Fortun, J., W.A. Dunn, S. Joy, J. Li, and L. Notterpek. 2003. Emerging role for autophagy in the removal of aggresomes in Schwann Cells. J. Neurosci. 23: 10672-10680.
3. Ellis,R.C., J.N. Earnhardt, R.L. Hayes, K.K. Wang, and D.K. Anderson. 2004. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J. Nuerochem. 88:689-697.
4. Kiepe D. et al.: Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I. Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.
PMID: 18556354
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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