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Product : Antibody >> Antigen Affinity Purified , Apoptosis , Loading Controls , Neurobiology , New Antibodies >> GAPDH - Loading Control
      
 

Peptide-affinity Purified Polyclonal Antibody to GAPDH - Loading Control

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3073
GAPDH - Loading ControlPurified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3073
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions – novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Cow, Dog, Drosophila, Human, Mouse, New World monkey, Pig, Rat
Predicted React: Xenopus
Host: Goat
Application: ELISA: >1:16000
IHC (paraffin): 0.3µg/ml
Western blot analysis: 0.01-0.1 ug/ml
Storage: Aliquot and store antibody at -20°C.
Recommended
Positive Control:
Most cell lines and tissues will be positive for GAPDH. 
 

Background
Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH, G3PDH or GPDH) is one of the key enzymes involved in glycolysis; it catalyzes the reversible oxidative phosphorylation of glyceraldehydes-3-phosphate. The GAPDH gene is constitutively and stably expressed at high levels in almost all tissues and cells, and as such is considered to be a “housekeeping” gene. Housekeeping proteins like GAPDH are useful as loading controls for western blots and protein normalization. 

Antigen
Peptide with sequence HQVVSSDFNSDT, from C Terminus of the GAPDH protein sequence according to NP_002037 was used as immunogen for this antibody.

Application Notes
A GAPDH antibody is a widely used loading control for quantitative Western blotting. It is highly conserved across species and antibodies to GAPDH typically have broad species reactivity.

Genebank Info (Protein): NP_002037
Gene ID: 2597
 
GAPDH - Loading Control Antibody

IHC staining of paraffin embedded Human Tonsil using GAPDH antibody at 0.3µg/ml. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. A) Staining of germinal center cells. B) Staining of endothelial cells.

GAPDH - Loading Control Antibody IMG-3073

Application of the GAPDH antibody as a loading control in Western blots. Total proteins from various mouse tissue lysates were normalized using the GAPDH polyclonal antibody at 0.05 ug/ml. Cat nos: Brain (40101), Heart (40102), Kidney (40104), Liver (40105), Lung (40106), Stomach (40110), Spleen (40109), Ovary (40108), and Testis (40111).

GAPDH - Loading Control

Application of the GAPDH antibody as a loading control in Western blots. Total proteins from various human tissue lysates were normalized using the GAPDH polyclonal antibody at 0.05 ug/ml. Cat nos: Kidney (40144), Liver (40145), Stomach (40152), and Testis (40150).

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Reference
1. Burke JR, Enghild JJ, Martin ME, Jou YS, Myers RM, Roses AD, Vance JM,Strittmatter WJ. Huntingtin and DRPLA proteins selectively interact with the enzyme GAPDH. Nat Med. 1996 Mar;2(3):347-50. PMID: 8612237
2. Fortun, J., W.A. Dunn, S. Joy, J. Li, and L. Notterpek. 2003. Emerging role for autophagy in the removal of aggresomes in Schwann Cells. J. Neurosci. 23: 10672-10680.
3. Ellis,R.C., J.N. Earnhardt, R.L. Hayes, K.K. Wang, and D.K. Anderson. 2004. Cathepsin B mRNA and protein expression following contusion spinal cord injury in rats. J. Nuerochem. 88:689-697.
4. Kiepe D. et al.: Defined carboxy-terminal fragments of insulin-like growth factor (IGF) binding protein 2 exert similar mitogenic activity on cultured rat growth plate chondrocytes as IGF-I. Endocrinology. 2008 Oct;149(10):4901-11. Epub 2008 Jun 12.
PMID: 18556354


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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