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Product : Antibody >> Cancer Antibodies , DNA Methylation & Repair >> ERCC1
      
 

Peptide-affinity Purified Polyclonal Antibody to ERCC1
 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-31152
ERCC1Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-31152
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat IgG
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Goat
Application: Western blot: 0.3-1µg/ml.
Storage: Store at -20°C.
Recommended
Positive Control:		 A431 lysate
 

Background
Principal Names: excision repair protein, excision repair cross-complementing 1, UV20, COFS4, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), ERCC1 Official Symbol: ERCC1 Accession Number(s): NP_973730.1; NP_001974.1 Human GeneID(s): 2067 Important Comments: This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).

Antigen
Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1; NP_001974.1.

Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1µg/ml.

Genebank Info (Protein)
NP_973730.1; NP_001974.1

 
ERCC1 Antibody

Western blot analysis of ERCC1 in A431 lysate (35µg protein in RIPA buffer) using IMG-31152 at 0.3µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.EB08190 (0.3µg/ml) staining of A431 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Reference
Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.

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