Product : Antibody >> Cancer Antibodies , DNA Methylation & Rep.. >> ERCC1

Peptide-affinity Purified Polyclonal Antibody to ERCC1

Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
ERCC1Purified, Peptide Affinity
0.1 mg
Catalog No: IMG-31152
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat IgG
Clone: N/A
Purification: Antigen Affinity Chromatography
Species: Human
Host: Goat
Application: Western blot: 0.3-1g/ml.
Storage: Store at -20C.
Positive Control:
A431 lysate

Principal Names: excision repair protein, excision repair cross-complementing 1, UV20, COFS4, excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence), ERCC1 Official Symbol: ERCC1 Accession Number(s): NP_973730.1; NP_001974.1 Human GeneID(s): 2067 Important Comments: This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1).

Peptide with sequence DPGKDKEGVPQPS-C, from the N Terminus of the protein sequence according to NP_973730.1; NP_001974.1.

Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.3-1g/ml.

Genebank Info (Protein): NP_973730.1, NP_001974.1
Gene ID: 2067
ERCC1 Antibody

Western blot analysis of ERCC1 in A431 lysate (35g protein in RIPA buffer) using IMG-31152 at 0.3g/ml. Primary incubation was 1 hour. Detected by chemiluminescence.EB08190 (0.3g/ml) staining of A431 lysate (35g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.



Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.






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