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Peptide-affinity Purified Polyclonal Antibody to ZYX |
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| Catalog No: |
IMG-31246 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat IgG |
| Clone: |
N/A |
| Purification: |
Antigen Affinity Chromatography |
| Species: |
Human |
| Predicted React: |
Dog |
| Host: |
Goat |
| Application: |
Western blot: 0.1-0.3µg/ml. |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
Human Peripheral Blood Mononucleocytes lysates |
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Background Principal Names: HED-2, ESP-2, zyxin, ZYX
Official Symbol: ZYX
Accession Number(s): NP_003452.1
Human GeneID(s): 7791
Important Comments: Reported variants represent identical protein: NP_003452.1, NP_001010972.1 |
Antigen
Peptide with sequence C-QQREKPRVQEKQH, from the internal region of the protein sequence according to NP_003452.1. |
Application Notes
Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 85kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 61.3kDa according to NP_003452.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.1-0.3µg/ml. |
Genebank Info (Protein)
NP_003452.1 |
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IMG-31246 (0.1µg/ml) staining of Peripheral Blood Mononucleocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
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Reference
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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