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Monoclonal Antibody to Human TLR3/CD283 |
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| Catalog No: |
IMG-315A |
| Contents: |
0.1 mg in 0.2 ml PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Isotype: |
Mouse IgG1, Kappa |
| Clone: |
40C1285.6 |
| Purification: |
Protein G Chromatography |
| Species: |
Dog, Human, Mouse |
| Host: |
Mouse |
| Application: |
Flow (Cell Surface): see Wong et al, 2007 for details
Flow (Intracellular): 2-4 ug/ 1x10^6 cells
IF/ICC: see Ranjith-Kumar et al, 2007 and
IHC (paraffin): (see Furrie et al, 2005)
IHC: (see Harii et al, 2005)
IP: 2 ug/ 1x10^6 cells
Western blot analysis: 1-3 ug/ml
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| Storage: |
Store at 4°C. For long-term storage, store at -20°C |
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Background
The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. |
Antigen
A synthetic peptide corresponding to amino acids 55-70 (VLNLTHNQLRRLPAAN) of human TLR3 was used as immunogen, Gen Bank no. gb|AAH59372.1| . Human TLR3 is an 870 amino acid protein. |
Application Notes
This antibody has tested negative by western blot in mouse intestine cell lysate. |
| Genebank Info (Protein): NP_003256 |
| Gene ID: 7098 |
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Western blot analysis of TLR3 in lysates from A) untransfected 293, B) 293 cells transfected with human TLR3 cDNA, C) human intestine, D) placenta, E) heart and F) ovary using TLR3 antibody at 3 ug/ml . IMGENEX's goat anti-mouse HRP conjugate (IMGENEX, 20101) was used as secondary. |
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Intracellular flow analysis of TLR3 in human monocytes using 0.5 ug of IMG-315A. Shaded histogram represents monocytes without antibody; green represents isotype control (IMGENEX, 20109); red represents anti-TLR3 antibody. Goat anti-mouse IgG1 PE conjugate (BD) was used as secondary. |
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IHC analysis of TLR3 in (A) human gut lumen (longitudinal section, transverse region) using IMG-315A at 10 ug/ml (Data courtesy Dr. Elizabeth Furrie, University of Dundee) and mouse spleen tissue using isotype control (B) and IMG-315A (C) at 5 mg/ml. |
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Reference
1. Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A. J Immunol 164(11):5998-6004 (2000).
2. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998).
3. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
4. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001)
5. Rock FL., Hardiman G., Timans JC., Kastelein RA. and Bazan JF. Proc. Natl. Acad. Sci. U.S.A. 95 (2), 588-593 (1998).
6. Muzio M, Polentarutti N, Bosisio D, Prahladan MK, Mantovani A. J Leukoc Biol 67(4):450-456 (2000).
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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