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Peptide-affinity Purified Polyclonal Antibody to Bim (BimAD, BimACD, and BimABCD isoforms) |
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| Catalog No: |
IMG-3164 |
| Contents: |
0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Isotype: |
Goat Ig |
| Clone: |
N/A |
| Purification: |
Antigen-Affinity Chromatography |
| Species: |
Human |
| Host: |
Goat |
| Application: |
Western blot analysis: 0.5-2ug/ml |
| Storage: |
Store at -20°C. |
Recommended
Positive Control: |
K562 |
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Background
Principal Names – BCL2L11; BAM; Bim; BOD; BCL2-like 11 (apoptosis facilitator); bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death Official Gene Symbol - BCL2L11GenBank Accession Number – NP_619528;NP_996886;NP_619529; LocusLink ID - 10018 (human)Gene Ontology terms - apoptosis; induction of apoptosis; membrane; membrane fractionThis antibody is expected to recognise 3 reported isoforms: BimAD, BimACD, and BimABCD. |
Antigen
Peptide with sequence FNAYYARRLEK, from C Terminus of the protein sequence according to NP_619528; NP_996886; NP_619529. |
Application Notes
Approx 23 kDa band observed in K562 lysates (predicted MW of 19, 9, 12 kDa according to NP_619528; NP_996886; NP_619529). |
Genebank Info (Protein)
NP_619527 |
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Detection of Bim in K562 cell lysate (RIPA buffer, 35 µg total protein per lane) using IMG-3164 (0.5 µg/ml). Detected by western blot using chemiluminescence. |
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Reference
1. Marani M, Tenev T, Hancock D, Downward J, Lemoine NR. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol. 2002 Jun;22(11):3577-89. PMID: 11997495
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Research purposes only.
Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.
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