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Product : Antibody >> Antigen Affinity Purified , Apoptosis , Bcl-2 Family Members in S.. , Cell Cycle >> Bim (BimAD, BimACD, & BimABCD isoforms)
      
 

Peptide-affinity Purified Polyclonal Antibody to Bim (BimAD, BimACD, and BimABCD isoforms)

 
Cat. No.DescriptionFormatUnitPrice(USD)QuantityBulk Quotation
IMG-3164
Bim (BimAD, BimACD, & BimABCD isoforms)Purified, Peptide Affinity
0.1 mg
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Catalog No: IMG-3164
Imgenex is now a part of Novus Biologicals! All orders, billing and shipments will be received from Novus. Please contact Novus directly for any questions novus@novusbio.com. To insure proper processing of you order, please insure that your purchase orders show Novus Biologicals as the vendor.
Contents: 0.1 mg of purified antibody. 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Isotype: Goat Ig
Clone: N/A
Purification: Antigen-Affinity Chromatography
Species: Human
Host: Goat
Application: Western blot analysis: 0.5-2ug/ml
Storage: Store at -20C.
Recommended
Positive Control:
K562
 

Background
Principal Names BCL2L11; BAM; Bim; BOD; BCL2-like 11 (apoptosis facilitator); bcl-2 interacting protein Bim; bcl-2-related ovarian death agonist; bcl-2 interacting mediator of cell death Official Gene Symbol - BCL2L11GenBank Accession Number NP_619528;NP_996886;NP_619529; LocusLink ID - 10018 (human)Gene Ontology terms - apoptosis; induction of apoptosis; membrane; membrane fractionThis antibody is expected to recognise 3 reported isoforms: BimAD, BimACD, and BimABCD.

Antigen
Peptide with sequence FNAYYARRLEK, from C Terminus of the protein sequence according to NP_619528; NP_996886; NP_619529.

Application Notes
Approx 23 kDa band observed in K562 lysates (predicted MW of 19, 9, 12 kDa according to NP_619528; NP_996886; NP_619529).

Genebank Info (Protein): NP_619527
Gene ID: 10018
SwissProt: O43521
 
Bim (BimAD, BimACD, & BimABCD isoforms) Antibody

Detection of Bim in K562 cell lysate (RIPA buffer, 35 g total protein per lane) using IMG-3164 (0.5 g/ml). Detected by western blot using chemiluminescence.

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Reference
1. Marani M, Tenev T, Hancock D, Downward J, Lemoine NR. Identification of novel isoforms of the BH3 domain protein Bim which directly activate Bax to trigger apoptosis. Mol Cell Biol. 2002 Jun;22(11):3577-89. PMID: 11997495


Research purposes only. Not for diagnostic or in vivo use. This product is guaranteed to perform as indicated on the datasheet for one year from the date of purchase.


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